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Environmental Science https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1442099954139-LT6G4C5M1P8XOTCBR3WH/iStock_000011708362Small.jpg Home - Proteomics Discovery Locate and analyze proteins in histological samples Proteomics Discovery https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1442099995320-25V3DSO5BVEF6PI2LML0/iStock_000004387285Small.jpg Home - Drug Development Obtain visual evidence of drug activity in subject tissue. Drug Development https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1442099953923-HQJEEI9QX3U5ZX9NBG9W/iStock_000014904788Small.jpg Home - Forensic Science Detect endogenous and exogenous evidence on surfaces. Forensic Science https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/ff7813e9-830c-411a-b5b8-dfcbf79a897d/AT+sprayer+tile.png Home - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/38cd85eb-a71d-417a-843f-b59d2e5ad4fb/M3%2B+Sprayer+Tile.png Home https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/84d04c2e-61d0-4876-8c67-46eac5242fd6/M5+Sprayer+Tile+2.png Home https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/ba1d1927-5d79-4d82-880c-835aab6f68cd/SubliMATE+Tile.png Home - Make it stand out Whatever it is, the way you tell your story online can make all the difference. http://www.htximaging.com/htx-technologies daily 0.75 2015-10-05 http://www.htximaging.com/request-a-quote daily 0.75 2018-02-05 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1442251805359-LCS4W2ESDQB652105DFC/Cool+Architecure+.jpg Request A Quote_Old SF http://www.htximaging.com/environmental-science daily 0.75 2019-08-13 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565727735478-MKOZ7UMQGPQ4B3ZMJ964/Website%2BBanner.jpg Environmental Science https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565726263105-5EMEWER3ZSEYQOYRCDC4/Website%2BImage.jpg Environmental Science - HTX AN-44 MSI of Moss, Fungus, and Cyanobacteria on Agar to Investigate Tripartite Metabolic Interactions Visualizing microbial interactions with MALDI MSI has provided many insights into important biological processes, including metabolic exchange, antibiotic resistance, and microbial competition. Here, we detail a complete experimental workflow for MALDI-MSI of microbial cultures grown on agar, wherein we investigate the different ecological roles of members of a peatland environment. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565726340180-WIFMYRGLXGANMHL36L7Y/Figure4.png Environmental Science - HTX AN-45 Profiling Lipidomic & Metabolic Changes of Barley Roots by MSI As plant roots are one of the first organs to encounter and respond to environmental pressures in the soil, such as salinity stress, profiling the metabolic changes that occur in saline-stressed roots is of critical importance in order to understand the consequences of increasing soil salinity. However, plant roots are complex structures with developmental zones characterized by distinct metabolic profiles. Here, we profile the metabolic differences between control and acute salt-stressed seminal barley roots in four different root areas. http://www.htximaging.com/forensic-science daily 0.75 2019-08-14 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1442433015783-A75P5KN9PF7PEHTTZH4Y/iStock_000014904788Small.jpg MALDI MSI Applications in Forensic Science https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564848352780-HFKZ2R78L1PTQYXLL0VA/Forensic+Page+Website+Image.png MALDI MSI Applications in Forensic Science - HTX Application Note #50 In one simple experiment, hundreds of compounds were able to be associated with five different fingerprints. This technology allows for forensic scientists to utilize a non-targeted approach to investigate trace evidence from a single fingerprint found at a crime scene while preserving the fingerprint pattern for suspect identification. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564848788461-P1ZQG44WAKCJ8KAEL7H8/Figure2.jpg MALDI MSI Applications in Forensic Science - HTX Application Note #51 MALDI-FTICR-MS imaging showed the presence of cocaine in hair samples, showing the use of MALDI-MS imaging in forensic toxicology. http://www.htximaging.com/maldimsi-proteomics daily 0.75 2023-12-27 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564951031365-NH59BI14BX6XDPMLFT4B/Figure%2B1.jpg MALDI MSI in Proteomics https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564854431739-LY1UQRL0TKPLBH6498H7/Figure+1.png MALDI MSI in Proteomics - HTX Application Note #38 Selected MALDI FTICR MS ion images of intact proteins from mouse kidney infected with S. aureus. MALDI FTICR IMS experiments are highly valuable for immunological biology given that post-translational modification of proteins (i.e. oxidation) play a key role in the immune response. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564852725766-DG079XSJKV6UVDHVLZM9/Figure+2.jpg MALDI MSI in Proteomics - HTX Application Note #39 MALDI IMS of intact proteins is of great relevance for biomedical research, since it provides spatial information of endogenous proteins, as well as their post-translational modifications. However, intact protein imaging faces a number of inherent challenges related to throughput, the sensitivity at high spatial resolution, rates of image acquisition, and molecular specificity and identification. Next-generation platforms such as ultra-high speed MALDI-TOF and high mass resolution MALDI FTICR mass spectrometers can override such limitations, improving protein acquisition rates by ten-fold, achieving 10 µm spatial resolution with high sensitivity, and resolving protein isotopes up to ~20 KDa. http://www.htximaging.com/drug-development daily 0.75 2019-08-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564952902627-ZSDJ2HYNW4YPQHZ21H4F/Figure5.png Drug Development https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564952731340-EIJAZINJRTK0CD61U0KO/Figure+4.jpg Drug Development - HTX Application Note #42 MALDI IMS can allow a better understanding of the molecular and cellular processes underlying the pathologic findings in pre-clinical drug development that could enable scientists to better anticipate and predict human risk and response to a candidate pharmaceutical. In the present study, we aimed to investigate the potential age sensitivity to dabrafenib-induced renal toxicity. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564952836287-ADMB15D6BMSYYC70UC39/Figure3.png Drug Development - HTX Application Note #47 http://www.htximaging.com/patented-spray-nozzle daily 0.75 2024-05-08 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1633985058314-W115I3SI4OZMFWMHHHF8/Screen+Shot+2021-10-11+at+4.44.08+PM.png Proprietary Heated Spray Nozzle HTX M3+ Sprayer with heated nozzle and integrated pump. http://www.htximaging.com/contact daily 0.75 2026-01-26 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1560874879831-YUUWYX8RJYW8RM5MFSPI/Contact+Us+Banner.jpg Contact Us https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1443223692291-8QTVY50K2SFT0A5ZF75L/image-asset.jpeg Contact Us http://www.htximaging.com/blank daily 0.75 2015-10-02 http://www.htximaging.com/thanks daily 0.75 2015-11-30 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1448912600978-DT94W25ORTX5J6LRQCYN/image-asset.png Thanks http://www.htximaging.com/htx-m5-sprayer daily 0.75 2025-11-03 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1496534263089-IR2SH89JLLFB8DTOV9UH/M5_BLL8869_Reduced.jpg HTX M5 Sprayer https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1496534890379-GWKWTRGUKE6BBT95HS4M/M5_BLL8891_Reduced.jpg HTX M5 Sprayer https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1496534493141-K5EL04K5PKA193031DOC/_BLL8887_Reduced.jpg HTX M5 Sprayer http://www.htximaging.com/htx-sepquant-dropletprobe daily 0.75 2025-11-03 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1496577108416-EZ77W2WMOMZ40KKZ1V0A/Screenshot+2017-06-04+07.50.53.png HTX SepQuant DropletProbe https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1496577100556-UQBBE8R5552I5D7X7MMR/Screenshot+2017-06-04+07.51.02.png HTX SepQuant DropletProbe https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1496577090271-W6YR9LYKQLS9CFD3WAOH/Screenshot+2017-06-04+07.51.14.png HTX SepQuant DropletProbe http://www.htximaging.com/heated-tray daily 0.75 2021-02-15 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1518019879083-VMGONRUA27UEYHHC9P7N/IMG_6709.JPG Heated Sample Tray (patent-pending) - App Note #41 Describes some of the first data acquired using the HTX M5 Sprayer heated sample tray, demonstrating improved MALDI image resolution. http://www.htximaging.com/consumer-library-1 daily 0.75 2019-08-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1542653542924-BWX18Q8FTAT3BYVL1TAR/Sublimator+Brains.png Application Notes https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1550088341135-EKYLS94FS82EM3ZAAYGU/Technical+Note+Image.png Application Notes https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1550088303111-7UUI1SMJGMTNUEC69GQ5/Technical+Note+Image.png Application Notes - App Note #53 Let it brie: Using the non-destructive SepQuant droplet Probe sampling system to analyze the free amino acid content of various cheeses https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1550088268002-7BMX6598WPJ1WSG0VGVN/Technical+Note+Image.png Application Notes - App Note #52 Understanding Primary Metastasis of Ovarian Cancer via Imaging Mass Spectrometry of a Novel 3D Tissue Explant and Cellular Coculture https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1550088200745-EU627K5CNYB72H3WDFAK/Technical+Note+Image.png Application Notes - App Note #51 Monitoring the Distribution of Drugs of Abuse and Medication in Hair by MALDI-FTICR-MS Imaging https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1550088157355-8SEDSMUVZKM8TUP3O4UM/Technical+Note+Image.png Application Notes - App Note #50 MALDI MSI of Fingerprints Reveals Beauty Product Preference at M4i https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1542650120760-7US7U1R8GVSAJJXPC9AN/Technical+Note+Image.png Application Notes - App Note #49 Improved Image Resolution and Signal Sensitivity of Small Molecules using the HTX Sublimator https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1542649998961-CWWOPU9M9UMDV57R95A0/Technical+Note+Image.png Application Notes - App Note #48 High-Speed and High-Resolution Mass Spectrometry Imaging using the New uMALDI Source and WREnS https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1542649691517-WFOHRRODYPLVOFCJM2X9/Technical+Note+Image.png Application Notes - App Note #47 App Note #47 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1534769226707-NZV6SO5JEC2B5032SVA8/Technical+Note+Image.png Application Notes - App Note #46 Combining MALDI Imaging and LESA SepQuant dropletProbe using SCiLS Lab https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1532110759741-UGWYFD4LUNAXLBNA0RHS/Technical+Note+Image.png Application Notes - App Note #45 Investigating Metabolic Changes in Salinity-Stressed Barley Roots https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1532110815875-AIYRKRVZLO20SM1MNUNK/Technical+Note+Image.png Application Notes - App Note #44 Mass Spectrometry Imaging of Moss, Fungus, and Cyanobacteria on Agar https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1532110854078-JU6JMFDNCRA5MDWJJD18/Technical+Note+Image.png Application Notes - App Note #43 Updated Enzymatic Digestion Protocol...Coming Soon!!! https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1523264588141-V5HXGDW5L6787D8W931T/Technical+Note+Image.png Application Notes - App Note #42 IMS to Investigate Age-Related Renal Toxicity of RAF Inhibitor, Dabrafebnib https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1517866092600-ARJPRKDCBH0HXJTEZR4M/Technical+Note+Image.png Application Notes - App Note #41 Effects of Tray Temperature on Matrix Deposition and IMS Image Quality https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1517854237153-0NRD297I7GRKQZLKHB0Q/image-asset.png Application Notes - App Note #40 Using MALDI IMS to Re-diBacterial Biofilm Heterogeneity https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1517854226704-B6UGBP3VQOA6CQSA4U8X/Technical+Note+Image.png Application Notes - App Note #39 MALDI FTICR: Spatial Proteomics of Cystic Fibrosis Lungs https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1517854217342-LRR8Y0ZJV53ZHG8NJ5NU/image-asset.png Application Notes - App Note #38 Linking m/z Protein Images with Proteomics data https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1518019250861-EJU8L9XX7J07XQ8NYBNJ/Technical+Note+Image.png Application Notes - App Note #37 SepQuant dropletProbe™️: Automated LESA Technology https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1517857067680-DCD6B5PO7XW30VB88OI5/Technical+Note+Image.png Application Notes - App Note #36 Spray Application of DHB on Dried Agar for MALDI Imaging of Microbial Cultures https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1517857024200-B88T0WSQW6PIEX4F2AIH/Technical+Note+Image.png Application Notes - App Note #35 High-Resolution MALDI MSI of Lipids in Rat Brain Tissue in + and - Mode https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1517856975331-Y21LRQW0GI897LQHXQPT/Technical+Note+Image.png Application Notes - App Note #34 Sublimation-like Results of Dry Matrix Application: IMS of Plant Metabolites https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1517852977193-IWYNHGP2O6CMNWHV3OO1/image-asset.png Application Notes - App Note #32 Analysis of Latent Fingermarks by MALDI IMS https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1517852962801-E8X88ML8WHUX2LF4SDTQ/Technical+Note+Image.png Application Notes - App Note #31 Matrix Deposition Optimization: Improve detection; Reduce delocalization https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1517852951026-SNJA9RUY992JDWLRIJJA/image-asset.png Application Notes - App Note #30 Whole Body Imaging: Localization of parent drugs and metabolites in tissues https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1517850876471-ZU0PEI2IFP5N5QCEQFOE/Technical+Note+Image.png Application Notes - App Note #29 MALDI MSI of Lipids in Rat Brain Tissue in Positive and Negative Mode https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1517850843571-YUCOW2LY9EX3NDUBEW24/Technical+Note+Image.png Application Notes - App Note #28 Tech https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1517850811248-AMLNIQH4KKIO2FXGJ4L6/Technical+Note+Image.png Application Notes - App Note #27 Demonstration of trypsin spray deposition for in-tissue digestion. http://www.htximaging.com/tm-sprayer daily 0.75 2025-11-03 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1518031697768-M8PRCB8UB1UXCT8R3X54/M3_BLL9021.jpg HTX TM-Sprayer https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1518032840824-J78GYWOWO6GANIG4TPAN/M3_BLL9021+-+Copy2.jpg HTX TM-Sprayer https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1518031779608-07D01NRTKELAITABPBQQ/just+software+.png HTX TM-Sprayer http://www.htximaging.com/webtolead-return-page daily 0.75 2021-02-10 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1530809628741-Z0M5T9ADR4B8F2NDJSD9/M3_BLL9024_Cutaway_2MB.jpg Web-to-Lead Return Page https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1530809718626-KUSBG5ZJ3RNAB4SJD4F7/maldi1.38.jpg Web-to-Lead Return Page https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1530809667290-HUBJLX8GV9OYMSC2O9QF/M5_BLL8870_Cutaway+1.5MB.jpg Web-to-Lead Return Page http://www.htximaging.com/brochures daily 0.75 2018-12-14 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1542651721569-MORCUWDQ5I63DAJ005WN/Sprayer_Brain.png Brochures https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1542651968063-UXL03ZQ3V5Y2IFQY0D1G/M3_BLL9021.jpg Brochures - HTX TM-Sprayer™ Sample Preparation System for MALDI Imaging Mass Spectrometry https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1542652285762-M9FM7OY57H8GRZBUDSFW/M5_BLL8879_reduced.jpg Brochures - HTX M5 Sprayer™ Advanced Sample Preparation System for MALDI Imaging Mass Spectrometry https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1542652932285-AS7OY8SLFC4WHIR6U4YK/2018-P00016.jpg Brochures - SepQuant dropletProbe™ Liquid Extraction Surface Analysis System https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1542651808859-5WLYF01KDWD2HJK0MGH7/maldi1.37.jpg Brochures - HTX Sublimator™ High-Speed Nano-Coating System http://www.htximaging.com/sepquant-dropletprobe-publications daily 0.75 2018-11-26 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1543246590389-SRJ4WZ0HAUZAFUS6VGKL/Website+Image.png SepQuant dropletProbe Publications http://www.htximaging.com/sepquant-dropletprobe-publications-1 daily 0.75 2018-11-26 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1542653280236-68XHRUIOCS1QOVBWGLCJ/Figure+4.jpg Copy of SepQuant dropletProbe Publications http://www.htximaging.com/htx-tm-sprayer-publications daily 0.75 2018-11-26 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1543247909182-DTWCE6UJFEVUYZGA833J/Sprayer+Banner+Photo.png HTX TM-Sprayer Publications http://www.htximaging.com/htxan52 daily 0.75 2021-02-15 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564685342769-YYMVBF0J1YKWCL1HHZ44/Undivided%2Bslide%2B-%2BCopy.jpg Ovarian Cancer MALDI Mass Spec Imaging https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564687187760-H709VICV9BZHHN8W08HQ/Figure1.png Ovarian Cancer MALDI Mass Spec Imaging Figure 1. Overview of work-flow for MALDI-MSI. (A) Ovaries from 16-18 day old female mice were extracted and maintained in warm media. (B) Ovaries are separated from bursa and halved immediately prior to plating. Explants are placed in the center of half of the wells of the 8-well chamber mounted to an ITO-coated glass slide. (C) 300 μL of various cell cultures prepared in 1% agarose are plated in each well. Each cell type was plated with an ovarian explant and as a pure cell culture. (D) The entire slide was covered with a lid and incubated for 4 days at 5% CO2 and 37°C. (E) The 8-well chamber is removed. The 1% agarose 3D cell cultures maintain their shape, ensuring that the cultures do not come into contact with one another. (F) The slide is dried in a 37°C oven for 4 hours and is rotated 90° every hour, resulting in flat and secure agarose plugs for sampling. (G) Slides were sprayed with matrix using the HTX TM-Sprayer. (H) Slides were imaged using a Bruker Autoflex MALDI-TOF MS. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564687571513-CHFQ2062D9AWCAMIAQMM/Table1JPEGRev2.jpg Ovarian Cancer MALDI Mass Spec Imaging Table 1. Names and descriptions of the 4 different cell lines used in this study. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564688598384-XTIW9G7OHWJX6NKSBHBA/Figure2.png Ovarian Cancer MALDI Mass Spec Imaging Figure 2. (A) Samples post-incubation; (B) Samples after sieved matrix application; (C) Samples postdesiccation; (D) Samples post-incubation; (E) Samples post-desiccation; (F) Samples after sprayed matrix application. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564689292673-P6WU58OE9CX4H7TRAU5R/Figure3.png Ovarian Cancer MALDI Mass Spec Imaging Figure 3. (A) Images of crystals sprayed onto agarose plugs post-desiccation using the TM-Sprayer. (B) Images of crystals sieved onto agarose plugs prior to desiccation. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564689508708-88VCNGJW72AIJVX4LU79/Table1JPEGRev3.jpg Ovarian Cancer MALDI Mass Spec Imaging Table 2. TM-Sprayer method parameters for precoat and post-desiccation spraying. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564689969563-S8KFYZ92D1U7HGIIFESR/Undivided+slide.png Ovarian Cancer MALDI Mass Spec Imaging Figure 4. m/z 104 and m/z 136 were two of the 33 m/z signals that were identified to be significantly higher in the ovarian explant and MOE PTENshRNA cells coculture than in any of the other 7 conditions. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564690590924-44WMIP8O0VB1R3FKNPSO/Figure5.png Ovarian Cancer MALDI Mass Spec Imaging Figure 5. (A) Optical image of divided well of an 8-well chamber; (B) m/z 112 and (C) m/z 144 are two signals that were clearly originated from the ovary explant and migrated through the agarose towards the MOE PTENshRNA cells. http://www.htximaging.com/htxan53 daily 0.75 2021-02-15 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564692163532-22X517YMWSY1QP9RT6IE/All+Cheese.jpg LESA Free Amino Acid Content of Cheese https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564784597957-KT4JEU9N0XWJAGPFCHS4/Workflow+Picture.png LESA Free Amino Acid Content of Cheese Figure 1. Overview of experimental workflow: (A) All cheese sections were sampled via the SeqQuant® dropletProbe, (B) the droplets were separated using an Agilent 1100 HPLC system with an in-line Waters PAH, C-18 column, (C) data were collected using an electrospray AB Sciex QTRAP® 5500, and (D) analyzed using MRM triggered MS/MS (data shown: MRM extracted chromatograms for proline (blue), valine (red), leucine (green), and phenyalanine (gray). https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564786092922-GA7TJ8U9X8XPVFAW5G7F/Figure3+for+Website.png LESA Free Amino Acid Content of Cheese Figure 2. Examples of quality control photos obtained for every sampling event by the SeqQuant® dropletProbe: (A) Before droplet disbursement, (B) during surface sampling, and (C) upon retraction of the droplet. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564786478485-O2SV4ZBJ7Z6LKDMXYEFS/Comte+Graph+Rev2.png LESA Free Amino Acid Content of Cheese Figure 3. The measured ln-transformed values of 17 amino acids across three replicate samples of the body of Comte cheese. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564786573740-92NGZ45GFISYK2U41V2X/Comte+Graph+Legend.png LESA Free Amino Acid Content of Cheese https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564787430582-TBAR7EKEH2X8SW7JIH7B/RindBody+Graph+Rev2.png LESA Free Amino Acid Content of Cheese Figure 4. The measured averages of ln-transformed values of the 7 amino acids that were significantly different in the rind versus the body region of the cheeses. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564787824904-SZETGK6YUF00A9QTIXJ3/Glycine_ANOVA.png LESA Free Amino Acid Content of Cheese https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564787896691-2V3MHXHM7UM5TVJO49OB/Serine_ANOVA.png LESA Free Amino Acid Content of Cheese https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564787955216-NFB5FRP0ZURL2M171CW3/As%5Bartoc_ANOVA.png LESA Free Amino Acid Content of Cheese https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564789776396-W2HJJP75FABGBRH6D6HO/Figure6+for+Website.png LESA Free Amino Acid Content of Cheese Figure 7. The SepQuant® dropletProbe software interface allowed for easy visualization of intrasample variability as it related to spatial features of the sample. (A) Proline was more abundant in the mushroom of the triple cream brie cheese with mushrooms than in the body of the cheese. (B) Valine was more abundant in the air-exposed hole of the Emmantal cheese than the inside. (C) Leucine was more in the inner rind than the outer rind of the gouda cheese. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564789539104-I72WJLGPYLDOTOYA3MQ0/Figure8_appnote.png LESA Free Amino Acid Content of Cheese Figure 7. The heatmap depiction of relative aspartic acid levels in different cheese samples. Aspartic acid has previously been indicated to be one of the amino acids that primarily composes casein proteins (1). http://www.htximaging.com/htxan51 daily 0.75 2021-02-15 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564791843371-6RYKB457Q1PXKJHVRPVJ/Figure2.jpg Monitoring the Distribution of Drugs of Abuse and Medication in Hair by MALDI-FTICR-MS Imaging https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564792245305-8KS40EVR0NTHEJZJFYDB/TABLE1.png Monitoring the Distribution of Drugs of Abuse and Medication in Hair by MALDI-FTICR-MS Imaging Table 1. Spray parameters for hair samples. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564792119076-OHDOO0H4O33LQ21ROXSP/Figure1.png Monitoring the Distribution of Drugs of Abuse and Medication in Hair by MALDI-FTICR-MS Imaging Figure 1. Optical images of hair samples before and after matrix deposition. (A) Longitudinal sectioned hair sample prior to matrix application showing the center of the hair sample. (B) Hair sample following matrix application showing the homogenous crystal coverage. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564793009143-H9NBNMUSAKL5P5UFFFQU/Figure2.png Monitoring the Distribution of Drugs of Abuse and Medication in Hair by MALDI-FTICR-MS Imaging Figure 2. MALDI-FTICR-MS imaging of cocaine user hair samples. A) Optical image of longitudinally sectioned hair samples following matrix application. B) MALDI-FTICRMS images showing the distribution of B) benzoylecognine at m/z 290.1369, C) cocaine at m/z 304.1536 and D) cocaethylene at m/z 318.1627. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564794918003-J6DA6O5RQ4XAPMNM7RHL/Figure3.png Monitoring the Distribution of Drugs of Abuse and Medication in Hair by MALDI-FTICR-MS Imaging http://www.htximaging.com/htxan50 daily 0.75 2021-02-15 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564843367916-0HW4DJH5RNCLZLJVU3B4/ActiveWorker1.jpg MALDI MSI of Fingerprints Reveals Beauty Product Preference at M4i https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564843739214-VZ1ZA8HNGBFBKQIHFN05/CHCA+Table+JPEG.jpg MALDI MSI of Fingerprints Reveals Beauty Product Preference at M4i Table 1. Spray parameters. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564843788653-A5YY2IKSCSPASMLEBT3Z/workflow.jpg MALDI MSI of Fingerprints Reveals Beauty Product Preference at M4i Figure 1. Two workflows for fingerprint imaging. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564844004968-4WJ3LWFP6TVVFCQSMM2P/sublimator_rapiflex.jpg MALDI MSI of Fingerprints Reveals Beauty Product Preference at M4i Figure 2. MALDI MSI analysis of fingerprints was able to distinguish between Subject 1 who used both a blue ink pen (m/z 478.3) and hand lotion (m/z 537.4) during the day versus Subject 2 who only used hand lotion (m/z 537.4). https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564844055154-M6WEQ95FQ6X4EXJJD7FP/figure_TMSPRAYER_SYNAPT.jpg MALDI MSI of Fingerprints Reveals Beauty Product Preference at M4i Figure 3. Three unique compounds were identified in the fingerprints of the three different subjects whose fingerprints were collected on a single glass slide: m/z 324.2 was identified as the green ink preferred by Subject 4; m /z 439.3 was identified as the hand cream favored by Subject 5; m/z 365.1 was identified as the shampoo used by Subject 6. Subject 5 was found to prefer a different brand of hand lotion than that used by Subjects 1 and 2. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564844782562-P6KYFUVANU0WRTFOA8PE/Screenshot+%2852%29.png MALDI MSI of Fingerprints Reveals Beauty Product Preference at M4i Figure 4. (A) m/z 691 was identified only in the fingerprints of subjects 4 and 6. (B) m/z 298.3 was also identified only in the fingerprints of subjects 4 and 6. (C) m/z 1142 was identified in all three subjects' fingerprints. It is possibly an exogenous lipid compound from the soap at M4i. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564844900606-5XLU4HD3E73J6DEF2O31/Fingerprint+Spectra2.png MALDI MSI of Fingerprints Reveals Beauty Product Preference at M4i Figure 5. Average spectrum of 200 spectra extracted from the general chromatogram. http://www.htximaging.com/htxan49 daily 0.75 2021-02-15 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564845689907-J8UAFJ0YIE7QZ8Y5B6HC/Sprayer_Brain.png Improved Image Resolution and Signal Sensitivity of Small Molecules using the HTX Sublimator https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564846009103-F0EM66TZE6HRUQF430Q5/Table1Jpeg.jpg Improved Image Resolution and Signal Sensitivity of Small Molecules using the HTX Sublimator Table 1. Spray parameters. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564846793721-D571NPE02HQRGOCTIBWA/Figure+1+for+Website.png Improved Image Resolution and Signal Sensitivity of Small Molecules using the HTX Sublimator Figure 1. Ion images of rat brain serial sections (12 μm) prepared using (A) the HTX TM-Sprayer and (B) the HTX Sublimator. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564846874264-S2OUDPT8GQWPMDUV4DRJ/Spectra_ShorterX.png Improved Image Resolution and Signal Sensitivity of Small Molecules using the HTX Sublimator Figure 2. Comparison of overall spectra obtained from serial rat brain sections prepared using the HTX TM-Sprayer (blue spectra) and the HTX Sublimator (red spectra). https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564847069837-6IXXP5VWGA9KDPBRVS46/Rat%2BIntestine.jpg Improved Image Resolution and Signal Sensitivity of Small Molecules using the HTX Sublimator Figure 3. Reconstructed ion images of bile acids in the lumen of the rat intestine. Samples were prepared using the HTX Sublimator. http://www.htximaging.com/htxan38 daily 0.75 2025-03-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564950824678-MMUF3JE27FJ31PQFGGY5/Figure%2B4_rev2.jpg Linking m/z Protein Images with Proteomics Data https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564946400239-9FS2AX5O72M79JVH8UO4/Figure+1+Neutrophils.jpg Linking m/z Protein Images with Proteomics Data Figure 1. Adapted from (3), inhibition of bacterial processes through Mn2+ chelation by calprotectin. At the site of infection, neutrophils deliver a “double hit” to S. aureus by releasing calprotectin (crosses), which chelates Mn2+ and Zn2+, thus sensitizing S. aureus to ROS generated by the neutrophil. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564946073540-W1M4OC1RZ4FBD3ZDPI5G/Table+1.jpg Linking m/z Protein Images with Proteomics Data Table 1. Spray parameters MALDI matrix deposition. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564947706414-1AGOQNTWDTJVX5J2OIMO/Figure+2+Spectra.jpg Linking m/z Protein Images with Proteomics Data Figure 2. MALDI FTICR IMS of intact proteins from mouse kidney tissue of wild-type (blue), or calprotectin-KO (pink), infected with S. aureus. Images were taken from a lesion where neutrophils accumulate, in positive ion mode. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564947365344-298JZA0NNIX6QYF7EDYG/Figure+3_rev2.jpg Linking m/z Protein Images with Proteomics Data Figure 3. Selected MALDI FTICR MS ion images of intact proteins from mouse kidney infected with S. aureus. m/z 8,564 (ubiquitin, yellow), m/z 4,963 (thymosin 4, green), m/z 5,653 (histone H4, grey), m/z 7,024 (histone H2A1, blue), m/z 10,164 (S100A8, red). ~75 µm spatial resolution. Acquisition time ~4 hrs. Scale bar 2 mm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564947185992-P9ZFKFSIQDVNMX9A5I5K/Figure%2B4_rev2.jpg Linking m/z Protein Images with Proteomics Data Figure 4. Selected MALDI FTICR MS ion images of (A) intact S100A8 (red, m/z 10,164) and (B) its oxidation product S100A8+4O (blue, m/z 10,228) from mouse kidney infected with S. aureus. (C) Shows the H&E stain, same as Fig 3A. (D) Two color overlay; m/z 10,164 (red), m/z 10,228 (blue). ~75 µm spatial resolution. Acquisition time 2 sec / pixel, total ~4 hrs. Scale bar 2 mm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564947582589-G3DNW4YW6D5G4497HR9G/Figure+5.jpg Linking m/z Protein Images with Proteomics Data Figure 5. LC-MS/MS results for S100A8 – M37O/C42O3. High-mass resolution bottom-up fragmentation data for the tryptic peptide shown above. http://www.htximaging.com/htxan39 daily 0.75 2025-03-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564948449309-FCHA7KTOBB14PTGUKU8Q/Figure%2B3.jpg Integrating Ultra-High Speed MALDI-TOF and MALDI FTICR IMS For Spatial Proteomics https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564949998042-0VLPALXOGT2BWV0GQFA5/Table1.jpg Integrating Ultra-High Speed MALDI-TOF and MALDI FTICR IMS For Spatial Proteomics Figure 1. Spray parameters for MALDI matrix deposition. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564949967746-DN7LS3PTOG9QSFFJK8A7/Figure+1.jpg Integrating Ultra-High Speed MALDI-TOF and MALDI FTICR IMS For Spatial Proteomics Figure 1. (A) Image of CF human lung with trichome staining prior to IMS acquisition. CF human lung at 30 µM using the RapifleX MALDI Tissuetyper, on linear positive ion mode. (B) Overlay: m/z 15,125.74 (hemoglobin, green), m/z 11,305.05 (histone H4, blue), m/z 10,095.5 (calcyclin, yellow). (C) Overlay: m/z 15,125.74 (hemoglobin, green), m/z 11,305.05 (histone H4, blue), m/z 10834.93 (S100A8, red), m/z 10,095.5 (calcyclin, yellow). (D) Overlay: m/z 15,125.74 (hemoglobin, green), m/z 11,305.05 (histone H4, blue), m/z 3450 (neuthrophil defensing 1, red), m/z 10,095.5 (calcyclin, yellow). Pixels are beam scanned with a 30 x 30 pixel. 141,000 pixels were acquired in about 1.5 hrs. Scale bar 1mm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564950290127-WXNAA98M9589DAGKXFCA/Figure+2.jpg Integrating Ultra-High Speed MALDI-TOF and MALDI FTICR IMS For Spatial Proteomics Figure 2. High-spatial resolution (10 µm) MALDI-TOF IMS data of CF human lung, using the Bruker rapifleX MALDI Tissuetyper, in linear positive ion mode. Overlay: m/z 15,125.74 (hemoglobin, green), m/z 11,305.05 (histone H4, blue), m/z 10834.93 (S100A8, red), m/z 10,095.5 (calcyclin, yellow). Pixels are beam scanned with a 30 x 30 pixel (140,775 pixel size). https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564950593228-IE50X5V0PZPH2V7XVIR8/Figure+3.jpg Integrating Ultra-High Speed MALDI-TOF and MALDI FTICR IMS For Spatial Proteomics Figure 3. A) Trichrome staining of a clear cell renal cell carcinoma human sample. (B) and (C) show MALDI FTICR protein imaging data collected with a 15T FTICR Bruker SolariX, on positive ion mode, at 100 µm spatial resolution. Overlay for (B): m/z 5654.472 (histone H4 with an acetylation and demethylation, blue), m/z 4312.809 (turquoise), m/z 2400.582 (red), m/z 7933.941 (hemoglobin subunit, green). Overlay for (C): m/z 5654.472 (histone H4 with an acetylation and demethylation, blue), m/z 10097.28 (orange), m/z 2400.582 (red), m/z 7933.941 (hemoglobin subunit, green), m/z 4312.809 (pink). Acquisition time ~1.5 sec/pixel, total ~6 hrs. http://www.htximaging.com/htxan42 daily 0.75 2025-03-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564953838225-6W9WWHRFXDUX6JO0KCT4/Figure%2B3.jpg Utilizing MSI to Investigate Age- Related Renal Toxicity of RAF Inhibitor, Dabrafebnib https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564954181855-HF3SHOYQXMLSBF0I3NLN/Picture2.png Utilizing MSI to Investigate Age- Related Renal Toxicity of RAF Inhibitor, Dabrafebnib Table 1. Treatment conditions of 10 experimental groups. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564843739214-VZ1ZA8HNGBFBKQIHFN05/CHCA+Table+JPEG.jpg Utilizing MSI to Investigate Age- Related Renal Toxicity of RAF Inhibitor, Dabrafebnib Table 1. Spray parameters. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564954873358-B4J1V8R63VUDALW4FTU9/Figure+1.jpg Utilizing MSI to Investigate Age- Related Renal Toxicity of RAF Inhibitor, Dabrafebnib Figure 1. Optical scan of kidney tissue sections from juvenile rats: (a) Group 6 Rat 4, (b) Group 10 Rat 2, (c) Group 1 Rat 1. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564955392064-G40U5AB9SLIOSA13W65B/Figure+2.jpg Utilizing MSI to Investigate Age- Related Renal Toxicity of RAF Inhibitor, Dabrafebnib Figure 2. Optical scans of kidney tissue sections from PND 7-13 juvenile rats (a) Group 6 Rat 2 and (b) Group 6 Rat 3 analyzed by MALDI MSI in CASI mode (m/z 460-620) at 100 μm spatial resolution. (c) and (d) Respective ion images for CDAB (m/z 508.1083) and quantity (ng/g of tissue) predicted using tissue mimetic model. (e) Pre-analysis optical scan of tissue mimetic model cores with spiked concentration of CDAB labeled for each (f) ion image for CDAB (m/z 508.1083) from tissue model analyzed under the same conditions as the kidney tissue sections. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564955747585-FKWZ4WA7T59RAJ9AEWXA/Figure+3.jpg Utilizing MSI to Investigate Age- Related Renal Toxicity of RAF Inhibitor, Dabrafebnib Figure 3. (a) Optical scan of kidney tissue sections from Group 6 Rat 2 analyzed by MALDI IMS in CASI mode (m/z 460–620) at 25 μm spatial resolution. (b) Ion image for CDAB (m/z 508.1083). (c) Serial H&E. (d) 10× magnification of outlined region in optical scan. (e) Magnified view of CDAB ion image coregistered with optical scan. (f) Magnified view of CDAB ion image co-registered with H&E. (g) Histopathology annotated H&E https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564955973246-UKCVGVBK0ZNGCJHCL3I1/Figure+4.jpg Utilizing MSI to Investigate Age- Related Renal Toxicity of RAF Inhibitor, Dabrafebnib Figure 4. (a) Optical scan of kidney tissue sections from Group 6 Rat 4 analyzed by MALDI MSI in fullscan mode (m/z 100–1000) at 10 μm spatial resolution. (b) Ion image for [3DHB – H + Ca]+ (m/z 501.0339) in green, and glycerophosphocholine [M + K]+ (m/z 296.0659) in red. (c) Ion image for [2DHB – 2H + Ca]•+ (m/z 345.9999). (d) Ion image for [2DHB – O – 2H + Ca]•+ (m/z 330.0047). http://www.htximaging.com/htxan47 daily 0.75 2025-03-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564957121087-PCDSJX7PFM3DJTLY22SQ/Figure3.jpg Characterizing the Distribution of Anti-HIV Drug, Tenofovir, in Colorectal Tissue by Imaging MS https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564957715060-ABLG6YGKP957423005DY/Table+1.jpg Characterizing the Distribution of Anti-HIV Drug, Tenofovir, in Colorectal Tissue by Imaging MS Table 1. Spray parameters https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564958117128-9K9RV4SW9E6VSEA28TC9/image-asset.jpeg Characterizing the Distribution of Anti-HIV Drug, Tenofovir, in Colorectal Tissue by Imaging MS Figure 1. HTX TM-Sprayer in the lab of Dr. Namandje N. Bumpus at Johns Hopkins University School of Medicine. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564957847215-MAKTI9Y3IOXNBC1U762P/IMG_8449.JPG Characterizing the Distribution of Anti-HIV Drug, Tenofovir, in Colorectal Tissue by Imaging MS Figure 2. LTQ Orbitrap XLTM Hybrid Ion Trap Orbitrap Mass Spectrometer (ThermoFisher ScientificTM) in the lab of Dr. Namandje N. Bumpus at Johns Hopkins University School of Medicine. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564958326138-68BU3RHTMEF96MR5G0ZL/Figure3.jpg Characterizing the Distribution of Anti-HIV Drug, Tenofovir, in Colorectal Tissue by Imaging MS Figure 3. (A) H&E stain of colorectal tissue biopsy. MALDI MS ion image of TFV (m/z = 288.0860 Da) (B), TFV-DP (m/z = 448.0194 Da) (C), PC (34:1) (m/z = 760.5856 Da) (D), PC (16:0/OH) (m/z = 496.3403 Da) (E), and PC (36:2) (m/z = 786.6013 Da) (F). Spatial resolution for MALDI MS ion images was 50 μm. The highest signal intensity (100%) is represented by red and the lowest signal is represented by blue for each ion of interest. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564958440029-V6598OAGKK7RZ0EK83N9/Figure4.jpg Characterizing the Distribution of Anti-HIV Drug, Tenofovir, in Colorectal Tissue by Imaging MS Figure 4. MALDI MS ion images of TFV in colorectal tissue biopsies from 4 study partcipants (J001-J004) at two different doses and timepoints. Spatial resolution for MALDI MS ion images was 50 μm. The highest signal intensity (100%) is represented by red and the lowest signal is represented by blue for each ion of interest. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564958551973-LV9NECA7XABTY12YJ7HD/Figure5.png Characterizing the Distribution of Anti-HIV Drug, Tenofovir, in Colorectal Tissue by Imaging MS Figure 5. Histochemical staining of CD4+ T cells (A) and (B) CD11c dendritic cells in colorectal tissue biopsy sections. Brown color indicates positive staining. MALDI MS ion image of (C) TFV (m/z = 288.0860 Da) and (D) TFV-DP (m/z = 448.0194 Da). Spatial resolution for MALDI MS ion images was 50 μm. The highest signal intensity (100%) is represented by red and the lowest signal is represented by blue for each ion of interest. http://www.htximaging.com/htxan46 daily 0.75 2025-03-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565635907302-HMTIYEB8SOEA5QN6XDSG/LinkedIn%2BPhoto%2B1.jpg HTX AN-46 Combining MALDI Imaging and LESA SepQuant® dropletProbe https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565635641829-PKKGDHX38GX5MWR6LU40/Figure+1.png HTX AN-46 Combining MALDI Imaging and LESA SepQuant® dropletProbe Figure 1. Orthologous mass spectrometry imaging techniques using MALDI imaging (A - D) and the SepQuant® dropletProbe (A, E - G). A. Serial sections of tissue; B. HTX TM-Sprayer for MALDI matrix deposition; C. Bruker rapifleX MALDI mass spectrometer; D. SCiLS Lab MALDI images; E. Liquid microjunction extraction by the SepQuant® dropletProbe for LC-MS analysis; F. Bruker timsTOF mass spectrometer; G. SCiLS Lab heatmaps. http://www.htximaging.com/htx-an40 daily 0.75 2025-03-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565614170691-GJVCOAFQHP6SW9GMK27M/Website%2BBanner3.jpg HTX AN-40 Re-discovering Bacterial Biofilm Heterogeneity with MALDI Mass Spectrometry Imaging https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565615359469-3TZ52NIGJ960C7C6KLTJ/Figure1.jpg HTX AN-40 Re-discovering Bacterial Biofilm Heterogeneity with MALDI Mass Spectrometry Imaging Figure 1. P. aeruginosa biofilms pre- and post-wash. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565625244285-UI5R0EK76V35UUL24K67/Figure2.jpg HTX AN-40 Re-discovering Bacterial Biofilm Heterogeneity with MALDI Mass Spectrometry Imaging Figure 2. Summarized experimental work-flow for MSI of bacterial biofilms. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565625673870-DL5AP5USZIRGBDJSU512/Table1.png HTX AN-40 Re-discovering Bacterial Biofilm Heterogeneity with MALDI Mass Spectrometry Imaging Table 1. Spray parameters for the in vitro grown biofilms. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565626370127-KWLCV0UO1HPOERD772ZJ/Table2.png HTX AN-40 Re-discovering Bacterial Biofilm Heterogeneity with MALDI Mass Spectrometry Imaging Table 2. Spray parameters for the biofilms on the cystic fibrosis explants. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565626725615-9FZ4KUB69KO324V43ZBO/Figure3.jpg HTX AN-40 Re-discovering Bacterial Biofilm Heterogeneity with MALDI Mass Spectrometry Imaging Figure 3. (A) H&E histology of CF human lung (right lower lobe). The inflamed airspace is shown enlarged (inset, gram stained). Optical images obtained at x 20 magnification using a Leica SCN400 Brightfield Slide Scanner. (B) CF human lung at 50 µM using the RapifleX MALDI Tissuetyper, on linear positive ion mode. Overlay: m/z 4,132.222 (green), m/z 4,048.831 (pink), m/z 2,534 (orange), and m/z 2,451 (blue). 100 shots/pixel in 20 µm steps, 4537 positions. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565627115121-GZCBW3GANLBBEGC6NV5F/Figure4.jpg HTX AN-40 Re-discovering Bacterial Biofilm Heterogeneity with MALDI Mass Spectrometry Imaging Figure 4. Four different cross-sections of Pseudomonas aeruginosa strain PA14 biofilms, untreated (A) or exposed to calprotectin in the media (B). Gram-Safranin stained images (post-analysis) are presented on the left, and MALDI MSI signals with differential biofilm localization are shown on the right. Overlay: m/z 6051 (green), m/z 5618 (blue), m/z 9099 (red). MALDI images collected at 50 µm, 66,571 pixels. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565627381465-Q9WEWIUAAN8TX6YM6LPF/Figure5.jpg HTX AN-40 Re-discovering Bacterial Biofilm Heterogeneity with MALDI Mass Spectrometry Imaging Figure 5. Summarized work-flow for proteomics analysis of PA14 biofilms. Central channel (A) or nutrient deplete (edge) regions (B) were untreated / treated with buffer / or exposed to calprotectin, dissected and lysed (80% ACN, 5% formic acid, 400 µL bacterial protein extraction reagent) for protein extraction. Protein samples were separated in a 10% Novex Bis-Tris gel at 200V, 5 min. Stained gel bands were excised and subjected to in-gel reduction, alkylation and tryptic digestion. Peptides were sequenced on a Thermo LTQ MS and the resulting spectra were searched against the Uniprot PA14 database using Sequest. Data were compiled using Scaffold version 4.4.3 (8). (A) displays the number of proteins abundant in the biofilm center, (B) in biofilm edges, and (C) proteins that were equally distributed through the biofilm. http://www.htximaging.com/microbiology daily 0.75 2019-08-13 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564951031365-NH59BI14BX6XDPMLFT4B/Figure%2B1.jpg Microbiology https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565728697470-IZ5FHJVFTWEGYUWOUW3P/Figure7.jpg Microbiology - HTX Application Note #36 Homogenous Spray Application of DHB on Dried Agar for MALDI Imaging of Microbial Cultures We present a workflow for applying 2,5-dihydroxybenzoic acid (DHB) to dried agar slices for MALDI imaging of metabolites from bacterial colonies. The MALDI mass spectra acquired in the agar and the bacterial colony when matrix was applied using the HTX TM-Sprayer contained many more peaks than the spectra acquired when the matrix was applied via dry coating with a sieve. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565737639003-7FO41I1U2UPV5XUO23X5/Figure4.jpg Microbiology - HTX Application Note #40 - Re-discovering Bacterial Biofilm Heterogeneity with MALDI MSI The combination of MALDI MSI, LA-ICP IMS and proteomics provides a powerful tool for the study of protein profiles (i.e. identification and spatial localization) and metal homeostasis within a bacterial biofilm, granting us the opportunity to rediscover biofilm architecture and heterogeneity, which seems tightly correlated with patterns of nutrient availability. http://www.htximaging.com/htxan41 daily 0.75 2025-03-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565562256238-JEYJIQ36UHRYG8Z1QNS2/Figure3.jpg HTX AN-41 Effects of Tray Temperature on Matrix Deposition and Mass Spectrometry Imaging https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565564172074-I5MGRNY7JDCLBHZ8KJCS/Table1.png HTX AN-41 Effects of Tray Temperature on Matrix Deposition and Mass Spectrometry Imaging Table 1 . Experimental conditions tested for optimal matrix coating. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565564634786-YI7R1V65JQ2WBTFG98JD/Figure2.png HTX AN-41 Effects of Tray Temperature on Matrix Deposition and Mass Spectrometry Imaging Figure 1. Microscopic pictures of matrix coated slides. (a) 4x magnified on-tissue images; (b) 10x magnified on-tissue images; (c) 4x magnified off-tissue images; (d) 10x magnified off-tissue images. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565564415814-89WOC8MVGFZ0PBF6RLJY/Table2.png HTX AN-41 Effects of Tray Temperature on Matrix Deposition and Mass Spectrometry Imaging Table 2. Spray parameters. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565564883863-WZ5S2CQ1Y3233D5E46P3/Figure3.png HTX AN-41 Effects of Tray Temperature on Matrix Deposition and Mass Spectrometry Imaging Figure 2. MS images of lipid species detected on rat brain sections at each condition. The m/z values, tentative lipid identification and MSI results are listed. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565565722132-LNN5R0HO09BK6W5DS1P5/Spectra.png HTX AN-41 Effects of Tray Temperature on Matrix Deposition and Mass Spectrometry Imaging Figure 3. Spectra corresponding to a limited m/z range from each of the four experimental conditions tested. http://www.htximaging.com/htxan44 daily 0.75 2025-03-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565708148899-T0CPM59BTERFMYAP7Y1T/Figure1c.jpg HTX AN-44 MSI of Moss, Fungus & Cyanobacteria on Agar to Investigate Metabolic Interactions https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565708654001-6WEU1HKW8ZX5TSCRF1ZZ/Figure1a.jpg HTX AN-44 MSI of Moss, Fungus & Cyanobacteria on Agar to Investigate Metabolic Interactions Figure 1A. The tripartitre interation of the moss (Sphagnum fallax), nitrogen-fixing bacteria (Nostoc muscorum), and the fungus (Trizodia spp.) grown in a petri dish on 1.5% BG110 agar. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565708740650-YPRL9JZVI7Z6R7FSJELT/Figure1b.jpg HTX AN-44 MSI of Moss, Fungus & Cyanobacteria on Agar to Investigate Metabolic Interactions Figure 1B. The agar area of the tripartite interaction was excised from the petri dish and transferred onto an ITO-coated glass slide. Samples were dehydrated overnight at ambient temperature and ambient pressure before DHB MALDI matrix was applied using the HTX TM-Sprayer. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565709100941-5JN5SKCFYD5UJY15JP7U/Table1.png HTX AN-44 MSI of Moss, Fungus & Cyanobacteria on Agar to Investigate Metabolic Interactions Table 1. Spray parameters for MALDI matrix deposition. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565710127487-U2K8RLFHE3QWSJ19WUHD/Figure1c.jpg HTX AN-44 MSI of Moss, Fungus & Cyanobacteria on Agar to Investigate Metabolic Interactions Figure 2. SCiLS spectral segmentation of tripartite interaction https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565710323800-A2MU34RKMD7OIF7QI2YU/Figure4.jpg HTX AN-44 MSI of Moss, Fungus & Cyanobacteria on Agar to Investigate Metabolic Interactions Figure 3. Distribution of some metabolites involved in tripartite communication. Lateral resolution: 200 μm https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565709594479-JJNYBU8SGKLZHDBZJT6T/Figure3jpg.jpg HTX AN-44 MSI of Moss, Fungus & Cyanobacteria on Agar to Investigate Metabolic Interactions Figure 4. Tracking the metabolic routes of possible candidates of ion m/z 189.1598 in a spatial manner in order to identify which of two natural compounds it represents. Co-localization of ion m/z 266.0934 and m/z 189.1598 and absence of any co-localization between m/z 162.1125 and m/z 189.1598 indicate that the ion m/z 189.1598 with the molecular formula C9H20N2O2 is 7,8 diaminononaoate. Lateral resolution: 200 μm. http://www.htximaging.com/htxan45 daily 0.75 2025-03-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565715831627-9TB7MK86CENWJX18YTIM/Figure4.jpg HTX AN-45 Profiling Lipidomic & Metabolic Changes of Barley Roots https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565716197232-PSTIJOXUZM9FADEF9P4F/Figure1_HighRes.png HTX AN-45 Profiling Lipidomic & Metabolic Changes of Barley Roots Figure 1. (A) Unstained and (B) Toluidine Blue O stained longitudinal sections of barley seminal root showing the different zones of the barley root. The left root in each image was grown in standard nutrient medium and the right root in each image was grown in nutrient medium containing 150 mM NaCl. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565715686440-FD9IRWH95MDFM4DPHHVA/Table1.png HTX AN-45 Profiling Lipidomic & Metabolic Changes of Barley Roots Table 1. Spray parameters for MALDI matrix deposition. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565714742165-YGBH0CGLUQ5Y04S6QCT2/Updated+Figure+2.jpg HTX AN-45 Profiling Lipidomic & Metabolic Changes of Barley Roots Figure 2. Basic overview of MALDI MSI workflow. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565714938824-E1RBJZ6GZZ0WS06Q8NN5/Figure3.png HTX AN-45 Profiling Lipidomic & Metabolic Changes of Barley Roots Figure 3. Reconstructed ion images of four out of the eight PC lipid species found via MALDI-MSI to be decreased in salt stress (right) versus in control (left) samples. These findings were confirmed by LC-MS. Images were recorded with a scanning step size of 30 × 30 μm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565715025203-28UKSUTV1TAF1RQIQVT6/Figure4.png HTX AN-45 Profiling Lipidomic & Metabolic Changes of Barley Roots Figure 4. Reconstructed ion images of three GPC adducts found via MALDI-MSI to be decreased in salt stress (right) versus in control (left) samples. Images were recorded with a scanning step size of 30 × 30 μm. Control and salt treated images have been set to the same intensity scale and obtained from the same MALDI-MSI experiment. http://www.htximaging.com/htxan36 daily 0.75 2025-03-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565721634833-MZZU13OMO22UOFOFUCCV/Figure7.jpg HTX AN-36 Homogenous Spray Application of DHB on Dried Agar for MALDI Imaging of Microbial Cultures https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565721917958-FPQ9SGWCO5XXZD6IX445/Figure1.jpg HTX AN-36 Homogenous Spray Application of DHB on Dried Agar for MALDI Imaging of Microbial Cultures Figure 1. Bacterial colony grown on agar media. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565721991957-FMKOVTAY2FS4SU2SF4VO/Figure2.jpg HTX AN-36 Homogenous Spray Application of DHB on Dried Agar for MALDI Imaging of Microbial Cultures Figure 2. Agar before (left) and after (right) drying. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565722099199-TR9XW43FYX67RD5XE4M3/Figure3.jpg HTX AN-36 Homogenous Spray Application of DHB on Dried Agar for MALDI Imaging of Microbial Cultures Figure 3. Agar that has been drying too long. It has begun to crack and flake away from the glass slide. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565722493978-ZPSNWEFB3LGJKLDU18YG/Table1.png HTX AN-36 Homogenous Spray Application of DHB on Dried Agar for MALDI Imaging of Microbial Cultures Table 1. Spray parameters for MALDI matrix deposition. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565722706021-TAQUO67BM8GM93E6WZ8I/Figure4.jpg HTX AN-36 Homogenous Spray Application of DHB on Dried Agar for MALDI Imaging of Microbial Cultures Figure 4. High resolution image of DHB matrix crystal size and coverage on a glass slide when applied with the HTX TM-Sprayer compared to application via sieve. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565722838241-UALCC4610J54UNNCDBCI/Figure5.jpg HTX AN-36 Homogenous Spray Application of DHB on Dried Agar for MALDI Imaging of Microbial Cultures Figure 5. A bacterial colony on agar on a glass slide with matrix applied via dry sieve (left) and the HTX TM-Sprayer (right). https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565722615856-DVKU8TL9CH58INADYQX8/Figure6.jpg HTX AN-36 Homogenous Spray Application of DHB on Dried Agar for MALDI Imaging of Microbial Cultures Figure 6. Mass spectra of bacterial colony samples when matrix is applied via the HTX TM-Sprayer or sieve. Metabolites with m/z 100-2000 were acquired. The inlay zooms in on the higher mass region, m/z 1000-2000, where dry sieving does not generate as many peaks as the HTX TM-Sprayer. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1565723076200-AL5P1NEXY56DBD05KKM8/Figure7.jpg HTX AN-36 Homogenous Spray Application of DHB on Dried Agar for MALDI Imaging of Microbial Cultures Figure 7. (A) Optical image of the bacterial colony on agar with DHB applied via the HTX TM-Sprayer (top) or sieve (bottom). (B) TIC image comparing the DHB coverage of the two application methods. (C) Representative ion images of m/z 230.095, 365.985, 664.113, and 1191.348 comparing metabolite distribution when matrix is applied with the HTX TM-Sprayer vs. sieve. Scale bar = 2 mm, Intensity scale = low abundance (blue) to high abundance (red). http://www.htximaging.com/htx-request-a-quote daily 0.75 2026-01-20 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1729185574197-XDBVVVHSCA1SPZQDOUHQ/Screen+Shot+2024-10-17+at+1.19.08+PM.png Request a Quote http://www.htximaging.com/htx-an-35-maldi-mass-spectrometry-imaging-of-lipids-in-positive-and-negative-ion-mode daily 0.75 2025-03-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1582125439997-A8S6H5M7MJAURECECQ6F/Figure3.jpg HTX AN-35 MALDI Mass Spectrometry Imaging of lipids in positive and negative ion mode https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1582125698381-Z5Q2Z8JOMHJS5Q3TEXHM/Table1.jpg HTX AN-35 MALDI Mass Spectrometry Imaging of lipids in positive and negative ion mode Table 1. Spray parameters for MALDI MSI in both positive and negative mode. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1582126200803-EMMP6SLA3T3ZMIFR6VHR/Figure1_website.jpg HTX AN-35 MALDI Mass Spectrometry Imaging of lipids in positive and negative ion mode Figure 4. High resolution image of DHB matrix crystal size and coverage on a glass slide when applied with the HTX TM-Sprayer compared to application via sieve. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1582126333311-SKK89FCB6MCU5OULBKEG/Figure2_website.jpg HTX AN-35 MALDI Mass Spectrometry Imaging of lipids in positive and negative ion mode Figure 2. Representative mass spectra recorded on rat brain sections in (A) positive ion mode, CHCA matrix, cerebral cortex, (B) positive ion mode, CHCA matrix, corpus callosum (white matter), (C) negative ion mode, 9-AA matrix, corpus callosum (white matter), and (D) negative ion mode, 9-AA matrix, cortex. Many lipid peaks are visible in spectra. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1582126384017-P6EPTNSO3VFVBHEGUOLW/Figure3.jpg HTX AN-35 MALDI Mass Spectrometry Imaging of lipids in positive and negative ion mode Figure 3. MALDI MSI image of rat brain recorded in negative ion mode (9-AA matrix). Two color overlay, Red m/z 885.55 ([PI38:4-H]-) Green m/z 888.63 ([ST42:2-H]-). Pixel size: 50 µM, acquisition time ~15 h. Scale bar represents 2 mm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1582126431466-R2PX55XU5B0PKV2GXSY8/Figure4.jpg HTX AN-35 MALDI Mass Spectrometry Imaging of lipids in positive and negative ion mode Figure 4. MALDI MSI image of rat brain recorded in positive ion mode (CHCA matrix). Two color overlay, Red m/z 772.7 ([PC32:0+K]+); Green m/z 826.8 ([PE40:0+Na]+). Pixel size: 50 µM, acquisition time ~15 h. Scale bar represents 2 mm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1582126498266-58WWKSZYGCW1SI7IIITK/Figure5.jpg HTX AN-35 MALDI Mass Spectrometry Imaging of lipids in positive and negative ion mode Figure 5. MALDI MSI image of rat cerebellum recorded in negative ion mode (9-AA matrix), Three color overlay, Red m/z 885.55 ([PI38:4-H]-); Green m/z 888.63 )[ST42:2-H]-); Blue m/z 806.55 ([ST36:1-H]-). Pixel size 10 µm, acquisition time ~5 h. Scale bar represents 500 µm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1582126556803-OKUP1P0D9B98IW96QI69/Figure6.jpg HTX AN-35 MALDI Mass Spectrometry Imaging of lipids in positive and negative ion mode Figure 6. MALDI MSI image of rat cerebellum recorded in positive ion mode (CHCA matrix). Two color overlay, Red m/z 772.7 ([PC32:0+K]+); Green m/z 826.8 ([PE40:0+Na]+). Pixel size 10 µm, acquisition time ~5.5 h. Scale bar represents 500 µm. http://www.htximaging.com/htx-an-37-characterization-of-bioactive-secondary-metabolites-by-the-sepquant-dropletprobe daily 0.75 2025-03-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584386336344-O22IHI05HDAIJN8MZ68A/figure8_fungi.png HTX AN-37 Characterization of Bioactive Secondary Metabolites by the SepQuant dropletProbe https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584386704273-E9HZNYK4YHVW0DZOCB3T/Figure1.jpg HTX AN-37 Characterization of Bioactive Secondary Metabolites by the SepQuant dropletProbe Figure 1. (A) Photograph of Asimina triloba and magnification of the fruit. (B) Images of X. cubensis (G536) grown in a glass Petri dish and placed in a sterile plant tissue-cultivating container (Plant Con®). https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584384170531-OOZ1G9J1KLTVOEMPU29D/HPLCTable.jpg HTX AN-37 Characterization of Bioactive Secondary Metabolites by the SepQuant dropletProbe Table 1. HPLC parameters for all experiments. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584384342485-OVD4SQ2OEDYC03UH15PR/MSTable.jpg HTX AN-37 Characterization of Bioactive Secondary Metabolites by the SepQuant dropletProbe Table 2. MS parameters for all experiments. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584384899617-5R67I4SQAKKSE9GK57HI/Figure2.jpg HTX AN-37 Characterization of Bioactive Secondary Metabolites by the SepQuant dropletProbe Figure 2. (A) Locations of paw paw where the droplet-LMJ-SSP directly sampled seed (black), pulp (red), and twig (green) and the portions that were cross-sectioned: ovary (blue), leaf (yellow), and petal (purple). (B) The mass defect filtered chromatograms around annonacin; 603.4807 ± 100 Da with a mass defect of ±25 mDa. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584385197409-EDKXX15OV5IFDIOL89P6/Figure4_Fungi.png HTX AN-37 Characterization of Bioactive Secondary Metabolites by the SepQuant dropletProbe Figure 3. The spatial distribution of griseofulvin on X. cubensis (G536) grown on MEA at (A) 2.5 weeks and (B) 5.5 weeks of growth displaying the locations of guttate and stroma formations. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584385857399-AWGUQ95QZN4OQ19SEG45/Figure4.jpg HTX AN-37 Characterization of Bioactive Secondary Metabolites by the SepQuant dropletProbe Figure 4. The spatial distribution indicating the relative intensities of griseofulvin on fungal culture X. cubensis (G536) for the stroma, mycelium, and guttates at 5.5 weeks. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584385264439-U4BGAZMLH0PQ7VC81ZSC/figure1_fungi.png HTX AN-37 Characterization of Bioactive Secondary Metabolites by the SepQuant dropletProbe Figure 5. The structure of the antifungal agent, griseofulvin (1; red), from Xylaria cubensis (G536). The polyhydroxyanthraquinones (2–6) from Penicillium restrictum (G85) were grouped into two categories (purple and blue) based on their distributions in co-culture. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584385359555-P48IAD3F8VY38DB2A5Y3/figure6_fungi.png HTX AN-37 Characterization of Bioactive Secondary Metabolites by the SepQuant dropletProbe Figure 6. The spatial distribution of both groups of polyhydroxyanthraquinones on fungal isolates of P. restrictum at (A) 2.5 weeks and (B) 5.5 weeks. The color coding corresponds to the structures in Figure 5. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584385233762-69XNRT4MZEEN5CHP59MB/Figure5_fungi.png HTX AN-37 Characterization of Bioactive Secondary Metabolites by the SepQuant dropletProbe Figure 7. The spatial distribution of griseofulvin from X. cubensis (G536) while grown in co-culture with P. restrictum (G85) at (A) 2.5 weeks and (B) 3.5 weeks. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584385621985-E69BEB0T2IQZD9VPJXW9/figure7_fungi.png HTX AN-37 Characterization of Bioactive Secondary Metabolites by the SepQuant dropletProbe Figure 8. The spatial distribution of the (A) blue and (B) purple groups of polyhydroxyanthraquinones on fungal isolates of P. restrictum (G85) while grown in co-culture with X. cubensis (G536) at 2.5 weeks. The color coding corresponds to the structures in Figure 5. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584385792142-68ZYIRTXHIZK6Q96SRJO/Figure9.jpg HTX AN-37 Characterization of Bioactive Secondary Metabolites by the SepQuant dropletProbe Figure 9. The visible discoloration of P. restrictum (G85) while in co-culture with the griseofulvin-producer, X. cubensis (G536), at (A) 2.5 weeks and (B) 3.5 weeks. The discolored regions of P. restrictum (G85) are circled in red. The visible expansion of X. cubensis (G536) while in co-culture with P. restrictum (G85) at (C) 5.5 weeks and (D) 8 weeks. The guttates were attributed to X. cubensis (G536) due to the detection of griseofulvin. The stroma were attributed to X. cubensis (G536) since P. restrictum (G85) does not produce stroma. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584385746194-CYDRJX0PQFOP7FL742X9/figure8_fungi+-+Copy.png HTX AN-37 Characterization of Bioactive Secondary Metabolites by the SepQuant dropletProbe Figure 10. Heat map of griseofulvin (red) and the P. restrictum (G85) metabolites (blue group only; purple metabolites were undetectable) at (A) 3.5 weeks and (B) 5.5 weeks. The heights of the bars are relative to their intensity from the HRMS data. http://www.htximaging.com/a-novel-silver-nitrate-spraying-protocol-for-fast-and-affordable-silver-assisted-laser-desorption-ionization-imaging-mass-spectrometry daily 0.75 2021-02-15 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584559593058-J73BZI1A9FQQZ2FCM7D9/AppNote+Website+Header.jpg Silver Nitrate Spraying Protocol for Silver-Assisted LDI-IMS https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584559891388-R32YON6BX57QX2C96YUH/Figure1.png Silver Nitrate Spraying Protocol for Silver-Assisted LDI-IMS Figure 1. AgNO3 and AgAc Spectra. Image was made in mMass. The spectra were normalized to the highest peak (Ag3+) https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584560311497-MI2R8118FRA8W7F55WB2/Table1_forWebsite.jpg Silver Nitrate Spraying Protocol for Silver-Assisted LDI-IMS Figure 2. All tested spraying conditions, with final optimized conditions shows in bold. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584560473026-NC7ZNSRYYBGAV4T65POU/Figure2.jpg Silver Nitrate Spraying Protocol for Silver-Assisted LDI-IMS Figure 2. Mean total CHO signal vs. spray thickness plot used to determine the optimal thickness for AgNO3 spray coating. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584560612265-TWR65H08BANEED8EU3IA/Figure3.jpg Silver Nitrate Spraying Protocol for Silver-Assisted LDI-IMS Figure 3. Crystal size and signal intensity varied based on the organic solvent composition. For all 8 solvent compositions tested, an optical image of the silver nitrate salt crystals off-tissue is shown in the top cell, and total ion current normalized IMS results of the Ag3 cluster at m/z 323.7 on the brain tissue homogenate is shown on the bottom. Scale bars represent 100 µm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584560735230-H3KZ3QMLAFDHQYQDYJR2/Figure4.jpg Silver Nitrate Spraying Protocol for Silver-Assisted LDI-IMS Figure 4. Effects of concentration on total cholesterol signal and signal homogeneity. For all 4 TFA concentrations tested, an optical image of the silver nitrate salt crystals off-tissue is shown in the top cell, and total ion current normalized IMS results of the Ag3 cluster at m/z 323.7 on the brain tissue homogenate is shown on the bottom. Scale bars represent 100 µm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584561011752-UWCISSP2BKYGQC0JLRCP/Figure5.jpg Silver Nitrate Spraying Protocol for Silver-Assisted LDI-IMS Figure 5. Triplicate IMS analysis of mouse brain sections at 100 µm spatial resolution demonstrates the reproducibility of the silver spray deposition sample preparation method. The cholesterol (CHO) signal distribution from the triplicate sections are nearly identical, with greater intensity in the fiber tracts of the white matter and the midbrain. The silver deposition (Ag3+ signal) is homogeneous across the sections. Scale bars represents 1 mm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584561122248-YPI6NVER22JWAGQBJJJM/Glycine_ANOVA.png Silver Nitrate Spraying Protocol for Silver-Assisted LDI-IMS Figure 6. High-resolution silver- assisted IMS from a horizontal mouse brain section was performed at 50-, 20-, and 10-µm spatial resolution after spray deposition AgNO3. Above right is an optical image of the mouse brain section used with the regions that were analyzed overlayed with the IMS results of CHO in grayscale. Scale bars represent 1 mm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1584561256961-XDBZBR66RCJY18QA6Q7J/Figure7.jpg Silver Nitrate Spraying Protocol for Silver-Assisted LDI-IMS Figure 7. Mean spectra of the cerebellum region acquired at 100 µm spatial resolution from half brain sections after AgNO3 spray deposition (top) and silver sputtering (bottom) show similar species detected. http://www.htximaging.com/htx-an-56-mass-spectrometry-imaging-of-neuropeptides-in-crustacean-brain-tissue daily 0.75 2021-02-15 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1586807988452-7XJW5U35N26J96BYCMS1/Figure2.jpg HTX AN-56 Mass Spectrometry Imaging of Neuropeptides in Crustacean Brain Tissue https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1586806371793-B5OO6KBW32ILMUSTSBNI/Figure1.png HTX AN-56 Mass Spectrometry Imaging of Neuropeptides in Crustacean Brain Tissue Table 1. Spraying parameters for DHB matrix on crustacean brain tissue. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1586806582778-H4G2E3RZN1T1WI4MLY29/Figure1.jpg HTX AN-56 Mass Spectrometry Imaging of Neuropeptides in Crustacean Brain Tissue Figure 1. Comparison of the normalized signal intensity and average number of neuropeptides identified from the top three washing protocols compared with a control condition of no wash. The purple bars represent the number of neuropeptides identified, and the green dots represent normalized signal intensity across n = 3 technical replicates. It was found that the 50:50 H2O:EtOH maximized both the intensity and number of neuropeptides among the three washes and was selected for further experiments and was selected for further experiments. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1586806783627-4BPUCUK3A986DALVYVMG/Figure2.jpg HTX AN-56 Mass Spectrometry Imaging of Neuropeptides in Crustacean Brain Tissue Figure 2. Examples of MALDI MS images of various neuropeptides in response to the optimized wash protocol. (a) An optical image of a crustacean brain, outlined in white. (b) A few neuropeptides displayed a decrease in signal intensity in the washed crustacean brain tissue versus the control (e.g. RFamide GPFLRFamide (m/z 735.430)). (c) Some neuropeptides displayed no change in signal intensity in the washed crustacean brain tissue versus the control (e.g. RFamide GYSKNYLRF (m/z 1146.605)). (d & e) Many neuropeptides displayed a clear increase in signal intensity in the washed crustacean brain tissue versus the control (e.g. RFamide LDRNFLRFamide (m/z 1079.611) and RFamide SQPSMRLRFamide (m/z 1120.604)). Scale bar represents 1 mm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1586807172072-XDIO5ARAS4FIG40C2OJS/Figure3.jpg HTX AN-56 Mass Spectrometry Imaging of Neuropeptides in Crustacean Brain Tissue Figure 3. Examples of MALDI MS images of various neuropeptides in response to the three stress conditions tested. (a) An optical image of crustacean brains, outlined in white. (b) A few neuropeptides displayed a clear increase in signal intensity in the stressed crustacean brain tissue versus the control (e.g. RFamide AHKNFLRFamide (m/z 1031.590)). (c) Some neuropeptides displayed decreases in signal intensity iin the stressed crustacean brain tissue versus the control (e.g. RFamide LPGVNFLRFamide (m/z 1061.626)). Scale bar represents 1 mm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1586806992254-FA35411FH1R3CBGGPZH8/Table2.jpg HTX AN-56 Mass Spectrometry Imaging of Neuropeptides in Crustacean Brain Tissue Table 2. The number of neuropeptides with significant intensity changes between each stress condition and the control condition and between each individual stress condition. http://www.htximaging.com/htx-an-57-maldi-msi-of-an-organochlorine-pesticide-with-dctb-matrix daily 0.75 2021-02-15 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1593532832793-KQJNJVRIMYZ0JL8RG8WF/Figure%2B5_Final.jpg HTX AN-57 MALDI MSI of an Organochlorine Pesticide with DCTB Matrix https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1593530118644-39VYQKY4LD1PKK2VH993/Figure+1.jpg HTX AN-57 MALDI MSI of an Organochlorine Pesticide with DCTB Matrix Figure 1. General schematic of chlordecone hydrate detection by MALDI MSI and a representative spectrum of the typical isotopic pattern. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1593530932303-J2GQ01OS8YV6TAE7CGYX/Figure+2_Final.jpg HTX AN-57 MALDI MSI of an Organochlorine Pesticide with DCTB Matrix Figure 2. (A) Microscopic image of a liver from a mouse that was exposed to chlordecone at 8 mg/kg for 10 days then CCl4 at 0.1 mg/kg. prior to MALDI imaging, with areas of health and necrotic tissue annotated. (B) MALDI-TOF-TOF MS images of m/z 506.68, chlordecone hydrate, in a mouse liver in negative ion mode with DCTB matrix. Pixel size 80 µm. Scale bars are 500 µm.* https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1593531241067-54TA4P3EC0P4DSATMTFX/Figure+3_Final.jpg HTX AN-57 MALDI MSI of an Organochlorine Pesticide with DCTB Matrix Figure 3. (A) Images of H&E stained prostrates from rats that were exposed to chlordecone at 5 mg/kg/week for 1, 15 or 20 weeks with the tumor and stroma annotated. (B) MALDI FT-ICR MS images of the prostates in negative ion mode with DCTB matrix. Pixel size 80 µm. (C) Merged images of the H&E stained prostate and the MALDI FT-ICR MS images. It is clear that chlordecone hydrate accumulated in the tumor regions of the mouse prostate. Scale bars are 1 mm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1593530218723-TFFWUYEOU48BJNBGACSM/Table1.jpg HTX AN-57 MALDI MSI of an Organochlorine Pesticide with DCTB Matrix Table 1. Spraying parameters for DCTB matrix on rat prostate and testis tissues for the HTX M5 Sprayer. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1593531386440-TOE2I4QF6LP3CHAZOBES/Figure4_Rev2.jpg HTX AN-57 MALDI MSI of an Organochlorine Pesticide with DCTB Matrix Figure 4. MALDI FT-ICR MS images of chlordecone hydrate m/z 506.68 ± 10 Da in testis tissue from a rat that was exposed to chlordecone at 5 mg/kg/week for 15 weeks in negative ion mode with DCTB matrix prepared with the old sprayer device. Ion image is overlaid on a microscopic image. Pixel size 50 µm. Scale bar is 4 mm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1593531774244-LKF531T4ALZEWYN825PC/Figure+5_Final.jpg HTX AN-57 MALDI MSI of an Organochlorine Pesticide with DCTB Matrix Figure 5. (A) DCBT crystals on rat testis tissue prepared with the older spraying device. (B) DCBT crystals on rat testis tissue prepared with the HTX M5 Sprayer. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1593532004099-08UR00T2C6MRPXEMRWVE/Figure6.jpg HTX AN-57 MALDI MSI of an Organochlorine Pesticide with DCTB Matrix Figure 6. (A) MALDI FT-ICR MS images of chlordecone hydrate m/z 506.68 ± 10 Da in rat testis tissue in negative ion mode from a rat that were exposed to chlordecone at 5 mg/kg/week for 15 weeks in negative ion mode with DCTB matrix prepared with the HTX M5 Sprayer. Ion image is overlaid on a microscopic image. (B) Inset image at increased magnification in order to demonstrate the detection of chlordecone hydrate is distinctly visible in various substructures of testis. Pixel size 50 m.µm. Scale bar is 3 mm. http://www.htximaging.com/htx-an-58-a-novel-reactive-matrix-for-mass-spectrometry-imaging-of-neurotransmitters daily 0.75 2021-02-15 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1594494561112-8C694FP3Z5YSBHKY9XIF/Figure%2B4%2BPart%2B1.jpg HTX AN-58 A Novel Reactive Matrix for Mass Spectrometry Imaging of Neurotransmitters https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1594496714554-R4IJS22RVT5NABRQBB78/Tag-ONLOGO.jpg HTX AN-58 A Novel Reactive Matrix for Mass Spectrometry Imaging of Neurotransmitters https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1594494985235-178XILEDH5IY9P23ZW0I/Table1_D4.jpg HTX AN-58 A Novel Reactive Matrix for Mass Spectrometry Imaging of Neurotransmitters Table 1. Spraying parameters for the deposition of internal standards and FMP-10 onto tissue sections. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1594495335696-OVNT5UQSNRIZ202RQBWW/Figure1.jpg HTX AN-58 A Novel Reactive Matrix for Mass Spectrometry Imaging of Neurotransmitters Figure 1. Improved detection of neurotransmitters and their metabolites facilitated by on tissue derivatization. (a) The reaction between the FMP-10 reactive matrix and amines and phenolic hydroxyls. R1, R2 and R3 indicate variable substituents. (b) Optical image of a sagittal tissue section from a rat brain. (c–e) MALDI–MSI analysis facilitated by FMP-10 reactive matrix maps the distribution of (c) 5-HT, (d) DA, and (e) GABA. Logarithmic color scaling for ion intensity from 0-60% was used for 5-HT and DA while a linear color scale ion intensity from 0-100% was used for GABA. Images were acquired by MALDI FTICR– MS with lateral resolution of 50 μm. At each sampling position, 100 shots were used to acquire data for the m/z 150-1500 range. CP, caudate putamen; CX, cerebral cortex; HIP, hippocampus; HY, hypothalamus; LC, locus coeruleus; SPVC, spinal vestibular nucleus; STRv, ventral striatum; SNr, substantia nigra pars reticulata. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1594495489699-60LA3P3AZ6170X3DBDIL/Figure2.jpg HTX AN-58 A Novel Reactive Matrix for Mass Spectrometry Imaging of Neurotransmitters Figure 2. High lateral resolution MALDI-MSI analysis of rat brain tissue section. (a) Optical image of one hemisphere of a coronal rat brain tissue section. (b) Distribution of FMP-10 derivatized GABA, intensity scale 0-100%. (c) Distribution of FMP-10 derivatized DA, intensity scale 0-15%. Data were acquired by TOF-TOF MS (rapifleX, Bruker Daltonics) with lateral resolution of 10 μm. At each sampling position, 80 shots were used to acquire data for the m/z 300–900 range. Abbreviations: CTX, cerebral cortex; HIP, hippocampus; MB, midbrain; RAmb, midbrain raphe nuclei; SNr, Substantia nigra; VTA, ventral tegmental area. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1594495964165-0VJ9SH9GUFM9E16E28XY/Combined+Figure3.jpg HTX AN-58 A Novel Reactive Matrix for Mass Spectrometry Imaging of Neurotransmitters Figure 3. Identification of NTs and metabolites by MS/MS fragmentation of their FMP-10 derivatized species. MS/MS imaging experiments were conducted on rat brain tissue section. The MALDI MS/MS images show the distributions of product ions of FMP-10 derivatized (a) DA (double derivatized, m/z 674.28) and (b) NE (double derivatized, m/z 690.27). Upper panels represent average MS/MS spectra acquired on brain tissue sections, while lower panels show MS/MS spectra of standards spotted and derivatized on stainless steel plate. The ranges of the color intensity scale are 0-60% for all DA and NE precursors and fragments except for NE (m/z 690.27) where the intensity scale was set to 0-100% (a, b). MS/MS images were acquired using a MALDI FTICRMS (solariX, Bruker Daltonics). At each sampling position, 100 shots were used to acquire data. The isolation window was set to 1 Da and the collision voltage was 30 V. Scale bar, 5 mm; lateral resolution = 80 μm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1594496183552-EMHS4IH679R90KM5AEW1/Combined+Figure4.jpg HTX AN-58 A Novel Reactive Matrix for Mass Spectrometry Imaging of Neurotransmitters Figure 4. MALDI-MS images of NTs and metabolites acquired from control and PD disease model treated with L-DOPA. Imaging experiments were conducted on brain tissue sections from unilateral shamlesioned (a, right panel), unilateral 6- OHDA-lesioned (a, middle panel), and unilateral 6-OHDA-lesioned animals that were treated with sub-chronic daily LDOPA for 4 weeks, with a final dose of LDOPA- d3 (a, left panel). The subsequent MALDI-MSI images show the distributions of different NTs and metabolites in these three animals. The range of the color intensity scales and m/z values are presented in parenthesis. (b) DA (0-100%, 674.5), (c) 3-MT (0-100%, 702.5), (d) DOPAC (0-50%, 673.4), (e) NE (0-100%, 690.5), (f) NE (0-5%, 690.5), (g) EP (0-100%, 704.5), (h) 3-O-Methyldopa (0-100%, 479.3), (i) GABA (0-80%, 353.3), (j) 5-HT (0-100%, 444.4), (k) 5-HIAL (0-100%, 443.3), (l) 5- HTOL (0-100%, 445.3), (m) DA-d3 (0-100%, 677.5), (n) NE-d3 (0-60%, 693.5), (o) L-DOPA-d3 (0-10%, 468.3) and (p) 3-OMethyldopa- d3 (0-100%, 482.3). At each sampling position, 1800 shots were used to acquire data for the m/z 300–900 range. Data were acquired by TOF-TOF MS (rapifleX, Bruker Daltonics) with lateral resolution of 150 μm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1594496514017-TXTDH3YLBCEKRRGEA01O/Figure+5.jpg HTX AN-58 A Novel Reactive Matrix for Mass Spectrometry Imaging of Neurotransmitters Figure 5. Quantitative analysis of FMP-10 derivatized standard solutions of NTs. Calibration standard curves for DA and HVA. Deuterated DA and HVA standard solutions were spotted on brain tissue sections. Quantitative data were acquired using MALDI FTICR-MS (solariX, Bruker Daltonics) for DA as (a) single derivatized, DA as double derivatized with loss of (b) methyl and (c) proton as well as (d) single derivatized HVA. http://www.htximaging.com/htx-an-55-beyond-tissue-using-maldi-msi-to-investigate-protein-glycosylation-states-in-human-serum daily 0.75 2021-02-15 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1602615155646-L8RDRRMT5SDOA74BWO4S/Slide3.JPG HTX AN-55 Beyond Tissue: Using MALDI MSI to Investigate Protein Glycosylation States in Human Serum https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1602615302146-75GB8580TNFV0KJYTV1A/Slide1.JPG HTX AN-55 Beyond Tissue: Using MALDI MSI to Investigate Protein Glycosylation States in Human Serum Figure 1. Overview of MALDI MSI workflow. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1602615608699-9ZLEG6FRDV7TC8BANHAH/Table1.jpg HTX AN-55 Beyond Tissue: Using MALDI MSI to Investigate Protein Glycosylation States in Human Serum Table 1. Recipes for all solutions used in MALDI MSI antibody workflow. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1602615456167-Q4CTZE8X15R2OJBMQQX8/Slide2.JPG HTX AN-55 Beyond Tissue: Using MALDI MSI to Investigate Protein Glycosylation States in Human Serum Figure 2. (A) Diagram of 8-well modules attached to nitrocellulose-coated slides, (B) Image of 8-well modules attached to nitrocellulose-coated slides, (C) Validated antibodies spotted in 8-well modules on nitrocellulose-coated slides, (D) Dried antibody spots in 8-well modules on nitrocellulose-coated slides. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1602615723910-E0X62CGE3CGVDSCNT7PX/Table2.jpg HTX AN-55 Beyond Tissue: Using MALDI MSI to Investigate Protein Glycosylation States in Human Serum Table 2. Spraying parameters for enzyme and matrix deposition. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1602616688548-X1NWW7HJLV1KQIWOW0RF/Slide3.JPG HTX AN-55 Beyond Tissue: Using MALDI MSI to Investigate Protein Glycosylation States in Human Serum Figure 3. (A) MALDI MSI images of 3 N-glycans localized to immuocaptured proteins. (B) Example abundant N-glycans detected for each captured glycoprotein. (C) Quantification of two N-glycan signals on two different proteins in human serum from patients with and without cancer. http://www.htximaging.com/htx-m3-sprayer daily 0.75 2025-11-03 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/63670a34-da65-40a1-af42-40713a90ee82/_BLL1113-12x8.jpg HTX M3+ Sprayer https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1518032840824-J78GYWOWO6GANIG4TPAN/M3_BLL9021+-+Copy2.jpg HTX M3+ Sprayer https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1632246578787-W0STYWL4L7WIGWGARSK5/SoftwareInLaptopClipart.png HTX M3+ Sprayer http://www.htximaging.com/htxnext daily 0.75 2025-10-17 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1624384088259-PBWY8IZL0OQZKJWSEI6R/Headersnipped.PNG HTXnext https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/4d5d1a2d-0a7c-409b-a2d2-5daf86c4c8da/Screenshot+2025-10-17+at+1.49.28%E2%80%AFPM.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/fb92dfc4-e121-4da9-a4cf-78c259dc5281/Screen+Shot+2025-05-29+at+10.20.38+AM.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1494ff19-4dec-454a-a93c-52b2ae3e2921/Screen+Shot+2025-03-21+at+12.31.22+PM.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/01c17160-741e-4d9c-8713-d1949fc2500c/Headshots HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/ad5e9cb4-006d-484a-921c-6a7dee98530d/Screen+Shot+2024-12-12+at+4.27.03+PM.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/d09b583d-a27c-407c-91c7-7e63b453dd6c/Screenshot.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/d66609fb-5bf1-40cf-a6a2-0f53d47096d2/ScreenShot.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/c05c079e-02a7-47bd-bf92-7e0adb9ae715/Screen+Shot+2023-02-03+at+11.54.29+AM.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/0e40b681-f4d4-411a-818f-0924cae5a348/DecScreenShot.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/7462cc4a-3b6a-4f8b-b969-83fc1f19d3e9/Screen+Shot+2022-12-01+at+4.34.36+PM.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/f7a0f499-ee8c-49bc-aa67-5065c93bc631/Screen+Shot+2022-10-17+at+10.22.04+AM.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/97184315-e565-40cd-8984-889dc0f6ba09/Screen+Shot+2022-08-09+at+10.01.58+AM.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1d31d1c4-4468-4e9e-ba87-e78b6ad2ff49/Screen+Shot+2022-07-06+at+11.19.14+AM.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/c62d99ed-5c44-472e-b351-8ce3ccbbf43e/AspectScreenshot.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/da6f62ea-1aa4-4ac5-bbe3-87db89ef9f52/Vanderbilt+Screenshot.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/6dd35227-d994-4049-85d6-875fc5c48218/MUSC+Screenshot.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/4627fce1-bd78-414f-befd-f22c023f16d6/Screenshot+GSK+2.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1632424435776-RXHR7BK8MZMNZEZLOGYB/M4i+Screenshot.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1629989743329-687LVD9XY957SEIADIKS/HTXnext+UKY+Screenshot+2.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1626452541449-F3ITOUPBJ9ZZ5CHGL4XF/Screen+Shot+2021-07-16+at+11.47.57+AM.png HTXnext - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1629989890753-JZHUFAW3QXGWOIHKVVIX/UT+Screenshot+2.png HTXnext https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1629990050618-18OP9FESWA9ZEP344ZJS/PNNL+Screenshot+2.png HTXnext https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1624383948545-8SCDI6NAA13SL2ZK6EC6/image-asset.jpeg HTXnext http://www.htximaging.com/htx-an-58-a-novel-reactive-matrix-for-mass-spectrometry-imaging-of-neurotransmitters-1 daily 0.75 2021-10-11 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1594494561112-8C694FP3Z5YSBHKY9XIF/Figure%2B4%2BPart%2B1.jpg HTX AN-58 A Novel Reactive Matrix for Mass Spectrometry Imaging of Neurotransmitters (Copy) https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1594496714554-R4IJS22RVT5NABRQBB78/Tag-ONLOGO.jpg HTX AN-58 A Novel Reactive Matrix for Mass Spectrometry Imaging of Neurotransmitters (Copy) https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1594494985235-178XILEDH5IY9P23ZW0I/Table1_D4.jpg HTX AN-58 A Novel Reactive Matrix for Mass Spectrometry Imaging of Neurotransmitters (Copy) Table 1. Spraying parameters for the deposition of internal standards and FMP-10 onto tissue sections. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1594495335696-OVNT5UQSNRIZ202RQBWW/Figure1.jpg HTX AN-58 A Novel Reactive Matrix for Mass Spectrometry Imaging of Neurotransmitters (Copy) Figure 1. Improved detection of neurotransmitters and their metabolites facilitated by on tissue derivatization. (a) The reaction between the FMP-10 reactive matrix and amines and phenolic hydroxyls. R1, R2 and R3 indicate variable substituents. (b) Optical image of a sagittal tissue section from a rat brain. (c–e) MALDI–MSI analysis facilitated by FMP-10 reactive matrix maps the distribution of (c) 5-HT, (d) DA, and (e) GABA. Logarithmic color scaling for ion intensity from 0-60% was used for 5-HT and DA while a linear color scale ion intensity from 0-100% was used for GABA. Images were acquired by MALDI FTICR– MS with lateral resolution of 50 μm. At each sampling position, 100 shots were used to acquire data for the m/z 150-1500 range. CP, caudate putamen; CX, cerebral cortex; HIP, hippocampus; HY, hypothalamus; LC, locus coeruleus; SPVC, spinal vestibular nucleus; STRv, ventral striatum; SNr, substantia nigra pars reticulata. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1594495489699-60LA3P3AZ6170X3DBDIL/Figure2.jpg HTX AN-58 A Novel Reactive Matrix for Mass Spectrometry Imaging of Neurotransmitters (Copy) Figure 2. High lateral resolution MALDI-MSI analysis of rat brain tissue section. (a) Optical image of one hemisphere of a coronal rat brain tissue section. (b) Distribution of FMP-10 derivatized GABA, intensity scale 0-100%. (c) Distribution of FMP-10 derivatized DA, intensity scale 0-15%. Data were acquired by TOF-TOF MS (rapifleX, Bruker Daltonics) with lateral resolution of 10 μm. At each sampling position, 80 shots were used to acquire data for the m/z 300–900 range. Abbreviations: CTX, cerebral cortex; HIP, hippocampus; MB, midbrain; RAmb, midbrain raphe nuclei; SNr, Substantia nigra; VTA, ventral tegmental area. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1594495964165-0VJ9SH9GUFM9E16E28XY/Combined+Figure3.jpg HTX AN-58 A Novel Reactive Matrix for Mass Spectrometry Imaging of Neurotransmitters (Copy) Figure 3. Identification of NTs and metabolites by MS/MS fragmentation of their FMP-10 derivatized species. MS/MS imaging experiments were conducted on rat brain tissue section. The MALDI MS/MS images show the distributions of product ions of FMP-10 derivatized (a) DA (double derivatized, m/z 674.28) and (b) NE (double derivatized, m/z 690.27). Upper panels represent average MS/MS spectra acquired on brain tissue sections, while lower panels show MS/MS spectra of standards spotted and derivatized on stainless steel plate. The ranges of the color intensity scale are 0-60% for all DA and NE precursors and fragments except for NE (m/z 690.27) where the intensity scale was set to 0-100% (a, b). MS/MS images were acquired using a MALDI FTICRMS (solariX, Bruker Daltonics). At each sampling position, 100 shots were used to acquire data. The isolation window was set to 1 Da and the collision voltage was 30 V. Scale bar, 5 mm; lateral resolution = 80 μm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1594496183552-EMHS4IH679R90KM5AEW1/Combined+Figure4.jpg HTX AN-58 A Novel Reactive Matrix for Mass Spectrometry Imaging of Neurotransmitters (Copy) Figure 4. MALDI-MS images of NTs and metabolites acquired from control and PD disease model treated with L-DOPA. Imaging experiments were conducted on brain tissue sections from unilateral shamlesioned (a, right panel), unilateral 6- OHDA-lesioned (a, middle panel), and unilateral 6-OHDA-lesioned animals that were treated with sub-chronic daily LDOPA for 4 weeks, with a final dose of LDOPA- d3 (a, left panel). The subsequent MALDI-MSI images show the distributions of different NTs and metabolites in these three animals. The range of the color intensity scales and m/z values are presented in parenthesis. (b) DA (0-100%, 674.5), (c) 3-MT (0-100%, 702.5), (d) DOPAC (0-50%, 673.4), (e) NE (0-100%, 690.5), (f) NE (0-5%, 690.5), (g) EP (0-100%, 704.5), (h) 3-O-Methyldopa (0-100%, 479.3), (i) GABA (0-80%, 353.3), (j) 5-HT (0-100%, 444.4), (k) 5-HIAL (0-100%, 443.3), (l) 5- HTOL (0-100%, 445.3), (m) DA-d3 (0-100%, 677.5), (n) NE-d3 (0-60%, 693.5), (o) L-DOPA-d3 (0-10%, 468.3) and (p) 3-OMethyldopa- d3 (0-100%, 482.3). At each sampling position, 1800 shots were used to acquire data for the m/z 300–900 range. Data were acquired by TOF-TOF MS (rapifleX, Bruker Daltonics) with lateral resolution of 150 μm. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1594496514017-TXTDH3YLBCEKRRGEA01O/Figure+5.jpg HTX AN-58 A Novel Reactive Matrix for Mass Spectrometry Imaging of Neurotransmitters (Copy) Figure 5. Quantitative analysis of FMP-10 derivatized standard solutions of NTs. Calibration standard curves for DA and HVA. Deuterated DA and HVA standard solutions were spotted on brain tissue sections. Quantitative data were acquired using MALDI FTICR-MS (solariX, Bruker Daltonics) for DA as (a) single derivatized, DA as double derivatized with loss of (b) methyl and (c) proton as well as (d) single derivatized HVA. http://www.htximaging.com/htx-an59 daily 0.75 2021-10-12 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1633982686813-5JTTUBH9A4ALBRMU2JF3/Screen+Shot+2021-10-11+at+4.04.35+PM.png Sweet and Simple: Using HTX M3+ Sprayer for Streamlined Multi-Method Sprays for Glycan Analysis https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1634048561416-VLXLN54A0AEC1P3X6E2K/GP%2Bwebsite%2Blogo.jpg Sweet and Simple: Using HTX M3+ Sprayer for Streamlined Multi-Method Sprays for Glycan Analysis https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1633982966598-O4LOX3XACXGRT031D9NW/Figure1glycan.png Sweet and Simple: Using HTX M3+ Sprayer for Streamlined Multi-Method Sprays for Glycan Analysis - Make it stand out Figure 1. Automated PumpScripts attached to each spray method. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1633983206497-YF5GGK4TM5XUK1P80GP2/GlycanWorkflow.png Sweet and Simple: Using HTX M3+ Sprayer for Streamlined Multi-Method Sprays for Glycan Analysis - Make it stand out Figure 2. Workflow for N-glycan MALDI MSI analysis on FFPE tissues. Adapted from McDowell et al. (2021) Mass Spec Rev. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1633983395266-NX5ADZLSOQC9UCB7HBVT/Table2+Glycans.png Sweet and Simple: Using HTX M3+ Sprayer for Streamlined Multi-Method Sprays for Glycan Analysis - Make it stand out Table 1. Spraying parameters for sequential enzyme and matrix deposition by HTX M3+ Sprayer. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1633983608220-W9JQUMP1XWTRGMN0SW9R/GlycanSpectra.png Sweet and Simple: Using HTX M3+ Sprayer for Streamlined Multi-Method Sprays for Glycan Analysis Figure 3. Example overall spectrum from N-glycan tissue imaging. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1633983682547-DKN4R5VRA4QPYA8W4GZJ/Fig4+ProstateAB.png Sweet and Simple: Using HTX M3+ Sprayer for Streamlined Multi-Method Sprays for Glycan Analysis Figure 4. Prostate tumor tissue imaged at 40μm. (A) N-glycan localized to stroma surrounding tumor. (B) N-glycan localized to tumor regions. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1633983813576-3N2R9002ZDTMQJ0CMKRK/CalvinFigureUpdated.png Sweet and Simple: Using HTX M3+ Sprayer for Streamlined Multi-Method Sprays for Glycan Analysis Figure 5. Prostate tissue imaged at 40μm. (A) Trichrome staining of prostate tissue section. (B) HighmannoseN-glycanlocalizedtothelumenoftheprostatetissue. (C) Sialylated & fucosylated N-glycan localized to the regions of the tissue containing large amounts of collagen. (D) Overlay image of 3 specific N-glycans corresponding to 3 unique regions of the tissue section. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1633983932024-5SIN2BRBR998FKF5MKLI/Fig6Colon.png Sweet and Simple: Using HTX M3+ Sprayer for Streamlined Multi-Method Sprays for Glycan Analysis - Make it stand out Figure 6. Colon tumor tissue imaged at 20μm. (A) High mannose glycan localized to tumor region. (B) Sialylated & fucosylated glycan localized to surrounding smooth muscle tissue and crypts. (C) Overlay of 3 specific N-glycans corresponding to unique regions of the tissue. http://www.htximaging.com/an60 daily 0.75 2022-01-03 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1641234569476-PSV7TX6GU8OGDST5WGYG/Figure+3.png HTX AN-60 Forensic Analysis of Blood Fingermarks by Sequential Trypsin and Matrix Application https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1633982966598-O4LOX3XACXGRT031D9NW/Figure1glycan.png HTX AN-60 Forensic Analysis of Blood Fingermarks by Sequential Trypsin and Matrix Application - Make it stand out Figure 1. Automated PumpScripts attached to each spray method. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/021a336e-e8ca-4a47-8910-eecbc74d425b/Figure2.png HTX AN-60 Forensic Analysis of Blood Fingermarks by Sequential Trypsin and Matrix Application - Make it stand out Figure 2. Workflow for peptide MALDI MSI analysis on blood fingermarks. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/ee88bb28-996c-4417-bed1-b1496bcfaa63/Screen+Shot+2022-01-03+at+1.12.52+PM.png HTX AN-60 Forensic Analysis of Blood Fingermarks by Sequential Trypsin and Matrix Application - Make it stand out Table 1. Preparation protocols for all solutions used in MALDI MS blood fingermark peptide imaging workflow. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/6039de39-dbc5-4d8c-8d4d-d8ebfe727a0b/Screen+Shot+2022-01-03+at+1.12.39+PM.png HTX AN-60 Forensic Analysis of Blood Fingermarks by Sequential Trypsin and Matrix Application - Make it stand out Table 2. Spraying parameters for sequential enzyme and matrix deposition using the HTX M3+ Sprayer. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/ac374072-7866-41e0-8b2f-ffc6913ddbf7/Figure+3.png HTX AN-60 Forensic Analysis of Blood Fingermarks by Sequential Trypsin and Matrix Application Figure 3. Haem and blood specific peptides from a human blood fingermark following application of trypsin and CHCA matrix. Images shown are haem at m/z 616.17 (red), hemoglobin β at m/z 1149.56 (green), and apolipoprotein at m/z 1215.6 (blue). The 4th and last MS image from the left is a superimposition of these 3 species. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/b6410c2c-b771-42bc-990c-832676acd35a/Screen+Shot+2022-01-03+at+1.21.01+PM.png HTX AN-60 Forensic Analysis of Blood Fingermarks by Sequential Trypsin and Matrix Application Table 3. Key human blood peptides detected from blood fingermarks following tryptic digestion. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/8c0b16b8-9805-42ee-a244-fd86c78c6b00/Screen+Shot+2022-01-03+at+1.21.11+PM.png HTX AN-60 Forensic Analysis of Blood Fingermarks by Sequential Trypsin and Matrix Application - Make it stand out Figure 4. Example blood peptides detected from a human blood fingermark. Peptides shown are apolipoprotein A1 at m/z 1215.60 (ATEHLSTLSEK), thrombin at m/z 1194.63 (ELLESYIDGR), and complement 3 alpha at m/z 1065.54 (MDKVGKYP http://www.htximaging.com/htx-an-35-maldi-mass-spectrometry-imaging-of-lipids-in-positive-and-negative-ion-mode-1 daily 0.75 2025-03-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1641918426398-ZTKPVQ5LZ7ZOEFWDGGVO/Fig4b.png HTX AN-31 Improving Protein Detection and Reduction of Analyte Delocalization https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/6d332836-0ae3-48b4-b99b-e734c76917c8/Figure1.png HTX AN-31 Improving Protein Detection and Reduction of Analyte Delocalization - Make it stand out Figure 1. Ion intensity differences seen for lower molecular weight species (m/z 4,964) Representative spectra from rat kidney medulla showing signal intensity for the peak at m/z 4964, denoted with the asterisks, increasing from black spectrum (low - 10.8 a.u.) then red spectrum (high - 33.9 a.u.). Inset shows an expanded view of the peak of interest. Intensity axes were scaled to the same values. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/f9cce13c-55ee-4c01-bb68-c18204244f09/Figure2.png HTX AN-31 Improving Protein Detection and Reduction of Analyte Delocalization - Make it stand out Figure 2. Ion intensity differences seen for medium molecular weight species (m/z 8,570) Representative spectra from rat kidney medulla showing signal intensity for the peak at m/z 8570, denoted with the asterisks, increasing from 8.35 x 102 a.u. orange spectrum (low - 12.3 a.u.) to blue spectrum (high - 22.5 a.u.). Inset shows an expanded view of the peak of interest. Intensity axes were scaled to the same values. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/96cee60c-8c5d-431e-a9c5-ee6dccc2162d/Fig3.png HTX AN-31 Improving Protein Detection and Reduction of Analyte Delocalization - Make it stand out Figure 3. Ion intensity differences seen for higher molecular weight species (m/z 21,900) Representative spectra from rat kidney medulla showing signal intensity for the peak at m/z 21900, denoted with the asterisks, increasing from green spectrum (low - 1.2 a.u.) to purple spectrum (high - 1.4 a.u.). Inset shows an expanded view of the peak of interest. Intensity axes were scaled to the same values https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/70a1e76e-a28e-4ce4-ac52-15a70cc7d125/Fig4.png HTX AN-31 Improving Protein Detection and Reduction of Analyte Delocalization - Make it stand out Figure 4. Imaging comparison using rat kidney. Eight passes of SA (5 mg/mL) were applied using the TM Sprayer at varying temperature and velocity settings. (a) H&E stained serial rat kidney section (12 µm-thick). Scale bar represents 2 mm. (b) Composite ion images collected from 12 µm-thick rat kidney sections at 200 µm lateral resolution. Colors correspond to multiple m/z values: 4,748 & 4,964 (red); 6,052 & 6,084 (blue); and 8,570 & 14,218 (green). http://www.htximaging.com/htx-an61 daily 0.75 2022-02-07 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1644263145457-TH7CIEEAJUJD33TGKP4E/Fig5-cut.png HTX AN-61 Matrix Density Adjustments for AP/MALDI https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/a3d9c93f-c0bb-4a32-a8e7-10402b13c88b/Fig1.jpg HTX AN-61 Matrix Density Adjustments for AP/MALDI - Make it stand out Figure 1. (A) Overview of MassTech AP/MALDI Source. (B) AP/MALDI source attached to Thermo Orbitrap MS. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/b82ffe74-abc8-4cf1-89b3-94c34c738a75/Eq+1.png HTX AN-61 Matrix Density Adjustments for AP/MALDI - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/ab0fd3ac-8361-4e99-8b94-0762a3849de2/Table+1.png HTX AN-61 Matrix Density Adjustments for AP/MALDI - Make it stand out Table 1. Spraying parameters for matrix deposition using the HTX M5 Sprayer. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/6c54154f-1353-4835-b06a-a38bb14559c3/Figure2.png HTX AN-61 Matrix Density Adjustments for AP/MALDI Figure 2. Visual images of tissues coated with varying amounts of matrix. Matrix density for each section is shown above. (A) Tissue sections scanned with a flatbed scanner. (B) Tissue sections visualized with AP/ MALDI source camera. Black circles represent superimposed standard target plate, and each tissue image was stitched together from 20 individual images. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/546e2534-253b-41bd-9ec1-3cf0ff15ae40/Fig+3.jpg HTX AN-61 Matrix Density Adjustments for AP/MALDI Figure 3. Spectra from samples placed on ITO-coated slides receiving 1-4 passes of matrix. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/033b42ba-f6f9-4377-b915-13cfab929a56/Fig+4.jpg HTX AN-61 Matrix Density Adjustments for AP/MALDI - Make it stand out Figure 4. Spectra from samples placed on glass-coated slides receiving 1-4 passes of matrix. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/51e32777-bc2b-4a6d-9186-772f80ca556b/Fig5.png HTX AN-61 Matrix Density Adjustments for AP/MALDI - Make it stand out Figure 5. Effect of matrix density on imaging of metabolites on kidney sections. http://www.htximaging.com/an-28 daily 0.75 2025-03-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1666901622132-92ZH0C8O2JQL8R1PS8IZ/Screen+Shot+2022-10-27+at+4.12.38+PM.png HTX AN-28 MALDI Imaging of Metabolites in Root Nodule Tissue https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/622bbd1f-6795-431d-91ff-7bb5212fbb7f/Screen+Shot+2022-10-27+at+4.22.39+PM.png HTX AN-28 MALDI Imaging of Metabolites in Root Nodule Tissue - Make it stand out Figure 1. Methylene blue stained Medicago truncatula root nodule (Image courtesy of Dr. Jean-Michel Ané lab in the Department of Agronomy at UW-Madison.) https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/2482d8f2-af98-4748-99ab-d13e6b2cded7/Screen+Shot+2022-10-27+at+4.21.30+PM.png HTX AN-28 MALDI Imaging of Metabolites in Root Nodule Tissue - Make it stand out Table 1. Spraying parameters for matrix deposition using the HTX TM Sprayer. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/afd43aeb-2278-43ab-9a6e-c91fd61a2ff0/Screen+Shot+2022-10-27+at+4.29.54+PM.png HTX AN-28 MALDI Imaging of Metabolites in Root Nodule Tissue Figure 2. A) Overlaid images of m/z 342.44, 603.85, and 673.83 which show spatial differentiation in different parts of the root nodule. B) Distribution of m/z 342.44 localized to the nitrogen fixation zone region. C) Distribution of m/z 603.85 localized to the outer nodule region. D) Distribution of m/z 673.83 localized to the root region. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/04412db0-83cd-41ec-8636-fffba8afb60e/Screen+Shot+2022-10-27+at+4.31.04+PM.png HTX AN-28 MALDI Imaging of Metabolites in Root Nodule Tissue Figure 3. Reference Optical Image https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/79379591-5fd0-4303-b9e9-5747ce02e9f8/Screen+Shot+2022-10-27+at+4.32.17+PM.png HTX AN-28 MALDI Imaging of Metabolites in Root Nodule Tissue - Make it stand out Figure 4. Optical image of serial tissue sections before (left) and after (right) MALDI- MSI. The area of tissue that was imaged is outlined in red on the right. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/b596d6e1-b37a-48f8-812f-fa6198485900/Screen+Shot+2022-10-27+at+4.34.39+PM.png HTX AN-28 MALDI Imaging of Metabolites in Root Nodule Tissue - Make it stand out Figure 5. Zoomed in image of matrix crystals showing crystal size and coverage. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/0007e6f3-9638-48fb-ae23-9f2894c970fd/Screen+Shot+2022-10-27+at+4.35.28+PM.png HTX AN-28 MALDI Imaging of Metabolites in Root Nodule Tissue - Make it stand out Figure 6. Mass spectra for the three distinct regions of the root nodule tissue: outer nodule, nitrogen fixation zone, and root. Metabolites with m/z 80-1000 were imaged. The inlay zooms in on the region m/z 80-300 which has the greatest abundance of different metabolites. The calculated [M+H]+ for FMRFamide is 599.3, which was added to the matrix as an internal calibrant. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/a527ea24-0520-468f-8d93-5e1cc82ed4ea/Screen+Shot+2022-10-27+at+4.12.38+PM.png HTX AN-28 MALDI Imaging of Metabolites in Root Nodule Tissue - Make it stand out Figure 7. Medicago truncatula- intact plant before dissection (Image courtesy of Dr. Jean-Michel Ané in the Department of Agronomy at UW-Madison.) http://www.htximaging.com/method-development-template daily 0.75 2022-11-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/02fb988b-7b24-482b-b93c-db0c93639351/Screen+Shot+2022-11-03+at+3.19.55+PM.png Method Development Template - The easy way to design & optimize your spray experiments! http://www.htximaging.com/htx-an30-demonstration-of-maldi-matrix-deposition-for-whole-animal-tissue-imaging daily 0.75 2025-03-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1688672929252-6DNDY8NROBG7P8S68YUP/Screen+Shot+2023-07-06+at+3.47.49+PM.png HTX AN-30 Demonstration of MALDI matrix deposition for whole animal tissue imaging. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/388eaab3-8066-4720-b516-a707171095bf/Optical+Image.png HTX AN-30 Demonstration of MALDI matrix deposition for whole animal tissue imaging. - Make it stand out Optical image of whole-rat section after completion of the MALDI spray coating using the optimized HTX TM-Sprayer protocol described herein. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/aab794ce-457e-4839-bc4b-32c22b504cd5/Table+1.png HTX AN-30 Demonstration of MALDI matrix deposition for whole animal tissue imaging. - Make it stand out Table 1. Spraying parameters for matrix deposition using the HTX TM Sprayer. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/aa718fcf-ea38-4e6b-b7f6-1199a4fb6c51/Figure+1.png HTX AN-30 Demonstration of MALDI matrix deposition for whole animal tissue imaging. - Make it stand out Figure 1. MALDI MS Image of internal standard. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/c1cac859-b4fc-4a4c-a9d1-6cf3bda16c70/Figure+2.png HTX AN-30 Demonstration of MALDI matrix deposition for whole animal tissue imaging. Figure 2. Representative Spectra of Internal Standard Response Throughout Image Run. Non-tissue pixels selected from A) top, B) middle, C) bottom of image https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/222907bd-da32-427a-affa-12d864aefccf/Figure+3.png HTX AN-30 Demonstration of MALDI matrix deposition for whole animal tissue imaging. Figure 3. MALDI MS Image of Olanzapine (stitched) (313.1517 m/z) https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/dbc05312-1c90-4893-aafd-addeb8397b89/Figure+4.png HTX AN-30 Demonstration of MALDI matrix deposition for whole animal tissue imaging. - Make it stand out Figure 4. MALDI MS Image of Oxidative Metabolite (stitched) (329.1484 m/z) https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/14e91d43-d59e-4052-9e83-e40ad58649d4/Figure+5.png HTX AN-30 Demonstration of MALDI matrix deposition for whole animal tissue imaging. - Make it stand out Figure 5. MALDI MS Image of N-desmethyl Metabolite (stitched) (299.1356 m/z) http://www.htximaging.com/htx-an48-highspeed-and-highresolution-msi-using-umaldi-source-and-wrens daily 0.75 2025-03-04 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1688757552647-8MMFG3A980853E9HJTP5/Figure5_Paper.jpg HTX AN-48 High-Speed and High-Resolution MSI using uMALDI Source and WREnS https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/c45b3c79-e4be-461a-9028-c48cf2c9e477/Figure1.png HTX AN-48 High-Speed and High-Resolution MSI using uMALDI Source and WREnS - Make it stand out Figure 1. Graphic illustration of the increased time needed to acquire MALDI ion images with lower spatial resolution. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/b82ffe74-abc8-4cf1-89b3-94c34c738a75/Eq+1.png HTX AN-48 High-Speed and High-Resolution MSI using uMALDI Source and WREnS - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/02e484fb-1ea6-4e0b-af5b-34a522d57989/Table+1.png HTX AN-48 High-Speed and High-Resolution MSI using uMALDI Source and WREnS - Make it stand out Table 1. Spraying parameters for norharmane matrix deposition using the HTX TM Sprayer. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/891051c5-450d-4639-8274-11ed835af80d/Table+2.png HTX AN-48 High-Speed and High-Resolution MSI using uMALDI Source and WREnS - Make it stand out Table 2. Spraying parameters for CHCA matrix deposition using the HTX TM Sprayer. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/61c0cd22-f683-4a92-bf94-cd1faa300b7c/Figure+2.png HTX AN-48 High-Speed and High-Resolution MSI using uMALDI Source and WREnS - Make it stand out Figure 2. (A) General MSI workflow incorporating sample handling and preparation; (B) Acquisition of half of a rat brain on either the first generation source of the Synapt G2-Si or the uMALDI using discrete or continuing raster mode leading to big variance in term of acquisition time from 3.5 h to 40 h; (C) The data processing takes 30 min and allows spatial visualization of compounds of interest;(D) According to the chosen workflow, the overall analytical time can vary between 4.5 h to 41 h. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/f7658e15-37af-4958-9ec3-63775d5a7c18/Figure+4-3.png HTX AN-48 High-Speed and High-Resolution MSI using uMALDI Source and WREnS Figure 3. Spectra acquired on the uMALDI source using different scan speeds. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/6ad38ec3-bee9-4252-a580-11e5f4143527/Figure+5-4.png HTX AN-48 High-Speed and High-Resolution MSI using uMALDI Source and WREnS - Make it stand out Figure 4. High spatial resolution MALDI-MSI of cartilage tissue. (A) OA cartilage stained with o-safranin, fast green, and hematoxylin. (B) MALDI image obtained at 15 µm spatial resolution using the uMALDI coupled to WReNS (20 pixels per second). SM (d18:1 16:0) [M+H]+ (m/z 703.57, red color) was expressed in the damaged superficial part of the tissue, PC (18:0 18:1) [M+Na]+ (m/z 810.60, yellow color) was specifically expressed for chondrocytes while DMPE (34:1) [M+H]+ (m/z 746.57, blue color) was mostly found in the matrix of the tissue. Arrows are pointing out the chondrocyte pellets. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/d99d5007-71c2-4766-be3f-d9d6a23a4c96/Figure+6-5.jpg HTX AN-48 High-Speed and High-Resolution MSI using uMALDI Source and WREnS - Make it stand out Figure 5. Comparison between the first generation source and the uMALDI source coupled to a SYNAPT HDMS G2-Si system for lipids imaging in positive mode on rat cerebellum (spot-mode). (A) MALDI experiment on rat cerebellum performed at 45 µm spatial resolution using the 1st generation source. (B) MALDI image acquired using the new designed uMALDI source with a defocus laser to perform experiment at 45 µm spatial resolution. (C) Image and spectrum obtained after use of the 1st generation source at 15 µm spatial resolution. (D) uMALDI image at 15 µm using a focus laser and spectrum extracted from the data. http://www.htximaging.com/htx-sublimate daily 0.75 2026-01-21 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/c28700a9-dce3-4de6-a50f-b08a342d7d3d/_BLL1082-12x8.jpg HTX SubliMATE https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/5574676f-8db3-4b86-8805-f03ad27a5ae1/ToPrint2.png HTX SubliMATE https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/cd27b4ce-380e-443c-995e-2a8c8e4cb4d5/_BLL1094-12x8.jpg HTX SubliMATE http://www.htximaging.com/proteomics-copy daily 0.75 2023-12-27 https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564951031365-NH59BI14BX6XDPMLFT4B/Figure%2B1.jpg Proteomics (Copy) https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564854431739-LY1UQRL0TKPLBH6498H7/Figure+1.png Proteomics (Copy) - HTX Application Note #38 Selected MALDI FTICR MS ion images of intact proteins from mouse kidney infected with S. aureus. MALDI FTICR IMS experiments are highly valuable for immunological biology given that post-translational modification of proteins (i.e. oxidation) play a key role in the immune response. https://images.squarespace-cdn.com/content/v1/55eae94ae4b08810efb9cd87/1564852725766-DG079XSJKV6UVDHVLZM9/Figure+2.jpg Proteomics (Copy) - HTX Application Note #39 MALDI IMS of intact proteins is of great relevance for biomedical research, since it provides spatial information of endogenous proteins, as well as their post-translational modifications. However, intact protein imaging faces a number of inherent challenges related to throughput, the sensitivity at high spatial resolution, rates of image acquisition, and molecular specificity and identification. 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