SepQuant dropletProbe Features Overview
Developed in conjunction with Oak Ridge National Laboratory, the SepQuant dropletProbe allows liquid surface extraction of analytes on surfaces and further analysis in an HPLC-MS detector. The technique allows a powerful alternative to Quantitative Whole Body Autoradiography (QWBA) and maximises the use of advanced mass spectrometry platforms for analytes detection.
First, the surface sample to be analyzed is mounted in a custom
sample tray. Then an optical image of the mounted sample is acquired
using a flatbed scanner controlled with the DropletProbe Premium©
software developed at the Oak Ridge National Laboratory
Schematic of the surface sampling process including (a)
determining syringe-to-surface distance, (b) the positioning of the
syringe needle 100-300 μm above a defined surface spot, (c)
dispensing a discrete volume of extraction solvent onto a surface
creating a liquid junction between the needle and the surface,
(d) dissolution of the analyte in the extraction solvent, e) the liquid
is drawn back into the syringe needle after a predefined extraction
time, and (f) loaded into the sample loop followed by (g) injection for
a consecutive HPLC-MS/MS analysis. Steps (c)-(e) may be repeated
to improve extraction efficiency.
Extraction liquid dispensed by the needle in locked position
on (a) a tissue biopsy sample and on (b) a brain thin tissue section.
Zoomed in view in inset in (b) shows of liquid microjunction between
the tip of the needle and the sample.